2019年7月
Osteoblastic lysosome plays a central role in mineralization.
Science advances
- 巻
- 5
- 号
- 7
- 開始ページ
- eaax0672
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1126/sciadv.aax0672
Mineralization is the most fundamental process in vertebrates. It is predominantly mediated by osteoblasts, which secrete mineral precursors, most likely through matrix vesicles (MVs). These vesicular structures are calcium and phosphate rich and contain organic material such as acidic proteins. However, it remains largely unknown how intracellular MVs are transported and secreted. Here, we use scanning electron-assisted dielectric microscopy and super-resolution microscopy for assessing live osteoblasts in mineralizing conditions at a nanolevel resolution. We found that the calcium-containing vesicles were multivesicular bodies containing MVs. They were transported via lysosome and secreted by exocytosis. Thus, we present proof that the lysosome transports amorphous calcium phosphate within mineralizing osteoblasts.
- リンク情報
-
- DOI
- https://doi.org/10.1126/sciadv.aax0672
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/31281900
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609213
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000478770400111&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1126/sciadv.aax0672
- ISSN : 2375-2548
- PubMed ID : 31281900
- PubMed Central 記事ID : PMC6609213
- Web of Science ID : WOS:000478770400111