2017年9月
Production of omega 3 fatty acids in marine cyanobacterium Synechococcus sp strain NKBG 15041c via genetic engineering
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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- 巻
- 101
- 号
- 18
- 開始ページ
- 6899
- 終了ページ
- 6905
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00253-017-8407-1
- 出版者・発行元
- SPRINGER
Omega-3 fatty acids (omega 3 FAs) have attracted attention because they have various health benefits for humans. Fish oils are currently major sources of omega 3 FAs, but a sustainable supply of omega 3 FAs based on fish oils is problematic because of the increasing demand. In this study, the production potential of a genetically engineered marine cyanobacterium, Synechococcus sp. strain NKBG 15041c, was examined as an alternative source of omega 3 FAs. A change in fatty acid composition of this cyanobacterium was successfully induced by the expression of a heterologous Delta 6-desaturase, and the transformants synthesized stearidonic acid, which the wild type cannot produce. As a result of optimization of culture conditions, maximal contents of stearidonic acid and total omega 3 FAs reached 12.2 +/- 2.4 and 118.1 +/- 3.5 mg/g, respectively. The maximal omega 3 FA productivity was 4.6 +/- 0.7 mg/(Lai...day). These are the highest values of the contents of stearidonic acid and omega 3 FAs in genetically engineered cyanobacteria reported thus far. Therefore, genetically engineered Synechococcus sp. strain NKBG 15041c may be a promising sustainable source of omega 3 fatty acids.
- リンク情報
- ID情報
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- DOI : 10.1007/s00253-017-8407-1
- ISSN : 0175-7598
- eISSN : 1432-0614
- Web of Science ID : WOS:000408229200005