論文

査読有り
2012年6月

Microfluidic extraction and stretching of chromosomal DNA from single cell nuclei for DNA fluorescence in situ hybridization

BIOMEDICAL MICRODEVICES
  • Xiaozhu Wang
  • ,
  • Shin-ichiro Takebayashi
  • ,
  • Evans Bernardin
  • ,
  • David M. Gilbert
  • ,
  • Ravindran Chella
  • ,
  • Jingjiao Guan

14
3
開始ページ
443
終了ページ
451
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10544-011-9621-8
出版者・発行元
SPRINGER

We have developed a novel method for genetic characterization of single cells by integrating microfluidic stretching of chromosomal DNA and fiber fluorescence hybridization (FISH). In this method, individually isolated cell nuclei were immobilized in a microchannel. Chromosomal DNA was released from the nuclei and stretched by a pressure-driven flow. We analyzed and optimized flow conditions to generate a millimeter-long band of stretched DNA from each nucleus. Telomere fiber FISH was successfully performed on the stretched chromosomal DNA. Individual telomere fiber FISH signals from single cells could be resolved and their lengths measured, demonstrating the ability of the method to quantify genetic features at the level of single cells.

Web of Science ® 被引用回数 : 9

リンク情報
DOI
https://doi.org/10.1007/s10544-011-9621-8
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000303871400002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s10544-011-9621-8
  • ISSN : 1387-2176
  • Web of Science ID : WOS:000303871400002

エクスポート
BibTeX RIS