論文

査読有り
2012年7月

Chromatin-interaction compartment switch at developmentally regulated chromosomal domains reveals an unusual principle of chromatin folding

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Shin-ichiro Takebayashi
  • ,
  • Vishnu Dileep
  • ,
  • Tyrone Ryba
  • ,
  • Jonathan H. Dennis
  • ,
  • David M. Gilbert

109
31
開始ページ
12574
終了ページ
12579
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.1207185109
出版者・発行元
NATL ACAD SCIENCES

Several 400- to 800-kb murine chromosome domains switch from early to late replication during loss of pluripotency, accompanied by a stable form of gene silencing that is resistant to reprogramming. We found that, whereas enhanced nuclease accessibility correlated with early replication genome-wide, domains that switch replication timing during differentiation were exceptionally inaccessible even when early-replicating. Nonetheless, two domains studied in detail exhibited substantial changes in transcriptional activity and higher-order chromatin unfolding confined to the region of replication timing change. Chromosome conformation capture (4C) data revealed that in the unfolded state in embryonic stem cells, these domains interacted preferentially with the early-replicating chromatin compartment, rarely interacting even with flanking late-replicating domains, whereas after differentiation, these same domains preferentially associated with late-replicating chromatin, including flanking domains. In both configurations they retained local boundaries of self-interaction, supporting the replication domain model of replication-timing regulation. Our results reveal a principle of developmentally regulated, large-scale chromosome folding involving a subnuclear compartment switch of inaccessible chromatin. This unusual level of regulation may underlie resistance to reprogramming in replication-timing switch regions.

Web of Science ® 被引用回数 : 46

リンク情報
DOI
https://doi.org/10.1073/pnas.1207185109
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000307538200063&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.1207185109
  • ISSN : 0027-8424
  • Web of Science ID : WOS:000307538200063

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