論文

査読有り
2013年

Development of a single-cell array for large-scale DNA fluorescence in situ hybridization

LAB ON A CHIP
  • Yingru Liu
  • ,
  • Brett Kirkland
  • ,
  • James Shirley
  • ,
  • Zhibin Wang
  • ,
  • Peipei Zhang
  • ,
  • Jacquelyn Stembridge
  • ,
  • Wilson Wong
  • ,
  • Shin-ichiro Takebayashi
  • ,
  • David M. Gilbert
  • ,
  • Steven Lenhert
  • ,
  • Jingjiao Guan

13
7
開始ページ
1316
終了ページ
1324
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1039/c2lc40364a
出版者・発行元
ROYAL SOC CHEMISTRY

DNA fluorescence in situ hybridization (FISH) is a powerful cytogenetic assay, but conventional sample-preparation methods for FISH do not support large-scale high-throughput data acquisition and analysis, which are potentially useful for several biomedical applications. To address this limitation, we have developed a novel FISH sample-preparation method based on generating a centimetre-sized cell array, in which all cells are precisely positioned and separated from their neighbours. This method is simple and capable of patterning nonadherent human cells. We have successfully performed DNA FISH on the single-cell arrays, which facilitates analysis of the FISH results with the FISH-FINDER computer program.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1039/c2lc40364a
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000315688500014&DestApp=WOS_CPL
ID情報
  • DOI : 10.1039/c2lc40364a
  • ISSN : 1473-0197
  • Web of Science ID : WOS:000315688500014

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