論文

査読有り
2013年12月

Murine esBAF chromatin remodeling complex subunits BAF250a and Brg1 are necessary to maintain and reprogram pluripotency-specific replication timing of select replication domains

EPIGENETICS & CHROMATIN
  • Shin-ichiro Takebayashi
  • ,
  • Ienglam Lei
  • ,
  • Tyrone Ryba
  • ,
  • Takayo Sasaki
  • ,
  • Vishnu Dileep
  • ,
  • Dana Battaglia
  • ,
  • Xiaolin Gao
  • ,
  • Peng Fang
  • ,
  • Yong Fan
  • ,
  • Miguel A. Esteban
  • ,
  • Jiong Tang
  • ,
  • Gerald R. Crabtree
  • ,
  • Zhong Wang
  • ,
  • David M. Gilbert

6
開始ページ
42
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/1756-8935-6-42
出版者・発行元
BIOMED CENTRAL LTD

Background: Cellular differentiation and reprogramming are accompanied by changes in replication timing and 3D organization of large-scale (400 to 800 Kb) chromosomal domains ('replication domains'), but few gene products have been identified whose disruption affects these properties.
Results: Here we show that deletion of esBAF chromatin-remodeling complex components BAF250a and Brg1, but not BAF53a, disrupts replication timing at specific replication domains. Also, BAF250a-deficient fibroblasts reprogrammed to a pluripotency-like state failed to reprogram replication timing in many of these same domains. About half of the replication domains affected by Brg1 loss were also affected by BAF250a loss, but a much larger set of domains was affected by BAF250a loss. esBAF binding in the affected replication domains was dependent upon BAF250a but, most affected domains did not contain genes whose transcription was affected by loss of esBAF.
Conclusions: Loss of specific esBAF complex subunits alters replication timing of select replication domains in pluripotent cells.

Web of Science ® 被引用回数 : 19

リンク情報
DOI
https://doi.org/10.1186/1756-8935-6-42
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000330071200001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1186/1756-8935-6-42
  • ISSN : 1756-8935
  • Web of Science ID : WOS:000330071200001

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