論文

査読有り
2015年12月

Direct Visualization of DNA Replication Dynamics in Zebrafish Cells

ZEBRAFISH
  • Kenji Kuriya
  • ,
  • Eriko Higashiyama
  • ,
  • Eriko Avsar-Ban
  • ,
  • Yutaka Tamaru
  • ,
  • Shin Ogata
  • ,
  • Shin-ichiro Takebayashi
  • ,
  • Masato Ogata
  • ,
  • Katsuzumi Okumura

12
6
開始ページ
432
終了ページ
439
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/zeb.2015.1151
出版者・発行元
MARY ANN LIEBERT, INC

Spatiotemporal regulation of DNA replication in the S-phase nucleus has been extensively studied in mammalian cells because it is tightly coupled with the regulation of other nuclear processes such as transcription. However, little is known about the replication dynamics in nonmammalian cells. Here, we analyzed the DNA replication processes of zebrafish (Danio rerio) cells through the direct visualization of replicating DNA in the nucleus and on DNA fiber molecules isolated from the nucleus. We found that zebrafish chromosomal DNA at the nuclear interior was replicated first, followed by replication of DNA at the nuclear periphery, which is reminiscent of the spatiotemporal regulation of mammalian DNA replication. However, the relative duration of interior DNA replication in zebrafish cells was longer compared to mammalian cells, possibly reflecting zebrafish-specific genomic organization. The rate of replication fork progression and ori-to-ori distance measured by the DNA combing technique were approximate to 1.4kb/min and 100kb, respectively, which are comparable to those in mammalian cells. To our knowledge, this is a first report that measures replication dynamics in zebrafish cells.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1089/zeb.2015.1151
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000366317400007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1089/zeb.2015.1151
  • ISSN : 1545-8547
  • eISSN : 1557-8542
  • Web of Science ID : WOS:000366317400007

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