論文

査読有り
2017年5月

Birth of cloned mice from vaginal smear cells after somatic cell nuclear transfer

THERIOGENOLOGY
  • Hiroki Kuwayama
  • ,
  • Yoshiaki Tanabe
  • ,
  • Teruhiko Wakayama
  • ,
  • Satoshi Kishigami

94
開始ページ
79
終了ページ
85
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.theriogenology.2017.02.012
出版者・発行元
ELSEVIER SCIENCE INC

Less invasive methods for donor cell collection will facilitate reproduction of wild animals using somatic cell nuclear transfer. Stages of the estrous cycle in mice have long been studies using somatic cells that can be collected from vaginal walls using cotton tipped swabs in a relatively non-invasive manner. In this study, we examined the feasibility of these cells as sources of nuclei for somatic-cell cloning using nuclear transfer. Estrous cycles generally comprise proestrus, estrus, metestrus, and diestrus stages. In the present experiments, more than 60% of cells were nucleated in vaginal smears from all but the estrus stage. However, after somatic-cell nuclear transfer of cells from proestrus, metestrus, and diestrus stages, 66%, 50%, and 72% of cloned embryos developed to the morula/blastocyst, and cloned female mouse birth rates after embryo transfer were 1.5%, 0.3%, and 1%, respectively. These results show that noninvasively collected vaginal smears contain somatic cells that can be used to clone female mice. (C) 2017 Published by Elsevier Inc.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1016/j.theriogenology.2017.02.012
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28407864
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000399860200011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.theriogenology.2017.02.012
  • ISSN : 0093-691X
  • eISSN : 1879-3231
  • PubMed ID : 28407864
  • Web of Science ID : WOS:000399860200011

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