論文

査読有り
2017年6月

Specific and spatial labeling of P0-Cre versus Wnt1-Cre in cranial neural crest in early mouse embryos

GENESIS
  • Guiqian Chen
  • ,
  • Mohamed Ishan
  • ,
  • Jingwen Yang
  • ,
  • Satoshi Kishigami
  • ,
  • Tomokazu Fukuda
  • ,
  • Greg Scott
  • ,
  • Manas K. Ray
  • ,
  • Chenming Sun
  • ,
  • Shi-You Chen
  • ,
  • Yoshihiro Komatsu
  • ,
  • Yuji Mishina
  • ,
  • Hong-Xiang Liu

55
6
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/dvg.23034
出版者・発行元
WILEY

P0-Cre and Wnt1-Cre mouse lines have been widely used in combination with loxP-flanked mice to label and genetically modify neural crest (NC) cells and their derivatives. Wnt1-Cre has been regarded as the gold standard and there have been concerns about the specificity of P0-Cre because it is not clear about the timing and spatial distribution of the P0-Cre transgene in labeling NC cells at early embryonic stages. We re-visited P0-Cre and Wnt1-Cre models in the labeling of NC cells in early mouse embryos with a focus on cranial NC. We found that R26-lacZ Cre reporter responded to Cre activity more reliably than CAAG-lacZ Cre reporter during early embryogenesis. Cre immunosignals in P0-Cre and reporter (lacZ and RFP) activity in P0-Cre/R26-lacZ and P0-Cre/R26-RFP embryos was detected in the cranial NC and notochord regions in E8.0-9.5 (4-19 somites) embryos. P0-Cre transgene expression was observed in migrating NC cells and was more extensive in the forebrain and hindbrain but not apparent in the midbrain. Differences in the Cre distribution patterns of P0-Cre and Wnt1-Cre were profound in the midbrain and hindbrain regions, that is, extensive in the midbrain of Wnt1-Cre and in the hindbrain of P0-Cre embryos. The difference between P0-Cre and Wnt1-Cre in labeling cranial NC may provide a better explanation of the differential distributions of their NC derivatives and of the phenotypes caused by Cre-driven genetic modifications.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1002/dvg.23034
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28371069
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000403362300004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/dvg.23034
  • ISSN : 1526-954X
  • eISSN : 1526-968X
  • PubMed ID : 28371069
  • Web of Science ID : WOS:000403362300004

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