論文

査読有り
2016年6月

Phenotypes of Aging Postovulatory Oocytes After Somatic Cell Nuclear Transfer in Mice

CELLULAR REPROGRAMMING
  • Ah Reum Lee
  • ,
  • Takashi Shimoike
  • ,
  • Teruhiko Wakayama
  • ,
  • Satoshi Kishigami

18
3
開始ページ
147
終了ページ
153
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/cell.2016.0014
出版者・発行元
MARY ANN LIEBERT, INC

Oocytes rapidly lose their developmental potential after ovulation, termed postovulatory oocyte aging, and often exhibit characteristic phenotypes, such as cytofragmentation, abnormal spindle shapes, and chromosome misalignments. Here, we reconstructed mouse oocytes using somatic cell nuclear transfer (SCNT) to reveal the effect of somatic cell-derived nuclei on oocyte physiology during aging. Normal oocytes started undergoing cytofragmentation 24 hours after oocyte collection; however, this occurred earlier in SCNT oocytes and was more severe at 48 hours, suggesting that the transferred somatic cell nuclei affected oocyte physiology. We found no difference in the status of acetylated alpha-tubulin (Ac-Tub) and alpha-tubulin (Tub) between normal and SCNT aging oocytes, but unlike normal oocytes, aging SCNT oocytes did not have astral microtubules. Interestingly, aging SCNT oocytes displayed more severely scattered chromosomes or irregularly shaped spindles. Observations of the microfilaments showed that, in normal oocytes, there was a clear actin ring beneath the plasma membrane and condensed microfilaments around the spindle (the actin cap) at 0 hours, and the actin filaments started degenerating at 1 hour, becoming completely disrupted and distributed to the cytoplasm at 24 hours. By contrast, in SCNT oocytes, an actin cap formed around the transplanted nuclei within 1 hour of SCNT, which was still present at 24 hours. Thus, SCNT oocytes age in a similar but distinct way, suggesting that they not only contain nuclei with abnormal epigenetics but are also physiologically different.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1089/cell.2016.0014
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27253626
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000377441000002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1089/cell.2016.0014
  • ISSN : 2152-4971
  • eISSN : 2152-4998
  • PubMed ID : 27253626
  • Web of Science ID : WOS:000377441000002

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