論文

査読有り
2016年4月

Generation of cloned mice and nuclear transfer embryonic stem cell lines from urine-derived cells

SCIENTIFIC REPORTS
  • Eiji Mizutani
  • ,
  • Kohei Torikai
  • ,
  • Sayaka Wakayama
  • ,
  • Hiroaki Nagatomo
  • ,
  • Yasuhide Ohinata
  • ,
  • Satoshi Kishigami
  • ,
  • Teruhiko Wakayama

6
開始ページ
23808
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep23808
出版者・発行元
NATURE PUBLISHING GROUP

Cloning animals by nuclear transfer provides the opportunity to preserve endangered mammalian species. However, there are risks associated with the collection of donor cells from the body such as accidental injury to or death of the animal. Here, we report the production of cloned mice from urine-derived cells collected noninvasively. Most of the urine-derived cells survived and were available as donors for nuclear transfer without any pretreatment. After nuclear transfer, 38-77% of the reconstructed embryos developed to the morula/blastocyst, in which the cell numbers in the inner cell mass and trophectoderm were similar to those of controls. Male and female cloned mice were delivered from cloned embryos transferred to recipient females, and these cloned animals grew to adulthood and delivered pups naturally when mated with each other. The results suggest that these cloned mice had normal fertility. In additional experiments, 26 nuclear transfer embryonic stem cell lines were established from 108 cloned blastocysts derived from four mouse strains including inbreds and F1 hybrids with relatively high success rates. Thus, cells derived from urine, which can be collected noninvasively, may be used in the rescue of endangered mammalian species by using nuclear transfer without causing injury to the animal.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1038/srep23808
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27033801
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000373202800001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/srep23808
  • ISSN : 2045-2322
  • PubMed ID : 27033801
  • Web of Science ID : WOS:000373202800001

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