論文

査読有り
2014年2月

Mid line-derived Shh regulates mesonephric tubule formation through the paraxial mesoderm

DEVELOPMENTAL BIOLOGY
  • Aki Murashima
  • ,
  • Hiroki Akita
  • ,
  • Mika Okazawa
  • ,
  • Satoshi Kishigami
  • ,
  • Naomi Nakagata
  • ,
  • Ryuichi Nishinakamura
  • ,
  • Gen Yamada

386
1
開始ページ
216
終了ページ
226
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ydbio.2013.12.026
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

During organogenesis, Sonic hedgehog (Shh) possesses dual functions: Shh emanating from midline structures regulates the positioning of bilateral structures at early stages, whereas organ-specific Shh locally regulates organ morphogenesis at later stages. The mesonephros is a transient embryonic kidney in amniote, whereas it becomes definitive adult kidney in some anamniotes. Thus, elucidating the regulation of mesonephros formation has important implications for our understanding of kidney development and evolution. In Shh knockout (KO) mutant mice, the mesonephros was displaced towards the midline and ectopic mesonephric tubules (MTs) were present in the caudal mesonephros. Mesonephros-specific ablation of Shh in Hoxb7-Cre;Shh(flox/-) and Sall1(CreERT2/+);shh(flox/-) mice embryos indicated that Shh expressed in the mesonephros was not required for either the development of the mesonephros or the differentiation of the male reproductive tract. Moreover, stage-specific ablation of Shh in Shh(CreERr2/flox) mice showed that notochord- and/or floor plate-derived Shh were essential for the regulation of the number and position of MTs. Lineage analysis of hedgehog (Hh)-responsive cells, and analysis of gene expression in Shh MO embryos suggested that Shh regulated nephrogenic gene expression indirectly, possibly through effects on the paraxial mesoderm. These data demonstrate the essential role of midline-derived Shh in local tissue morphogenesis and differentiation. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 14

リンク情報
DOI
https://doi.org/10.1016/j.ydbio.2013.12.026
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24370450
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000330258000019&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.ydbio.2013.12.026
  • ISSN : 0012-1606
  • eISSN : 1095-564X
  • PubMed ID : 24370450
  • Web of Science ID : WOS:000330258000019

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