論文

査読有り
2017年6月

Effect of Flooding and the nosZ Gene in Bradyrhizobia on Bradyrhizobial Community Structure in the Soil

MICROBES AND ENVIRONMENTS
  • Yuichi Saeki
  • ,
  • Misato Nakamura
  • ,
  • Maria Luisa T. Mason
  • ,
  • Tsubasa Yano
  • ,
  • Sokichi Shiro
  • ,
  • Reiko Sameshima-Saito
  • ,
  • Manabu Itakura
  • ,
  • Kiwamu Minamisawa
  • ,
  • Akihiro Yamamoto

32
2
開始ページ
154
終了ページ
163
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1264/jsme2.ME16132
出版者・発行元
JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE

We investigated the effects of the water status (flooded or non-flooded) and presence of the nosZ gene in bradyrhizobia on the bradyrhizobial community structure in a factorial experiment that examined three temperature levels (20 degrees C, 25 degrees C, and 30 degrees C) and two soil types (andosol and gray lowland soil) using microcosm incubations. All microcosms were inoculated with Bradyrhizobium japonicum USDA6(T), B. japonicum USDA123, and B. elkanii USDA76(T), which do not possess the nosZ gene, and then half received B. diazoefficiens USDA110(T)wt (wt for the wild-type) and the other half received B. diazoefficiens USDA110 Delta nosZ. USDA110(T)wt possesses the nosZ gene, which encodes N2O reductase; 110 Delta nosZ, a mutant variant, does not. Changes in the community structure after 30-and 60-d incubations were investigated by denaturing-gradient gel electrophoresis and an image analysis. USDA6(T) and 76(T) strains slightly increased in non-flooded soil regardless of which USDA110(T) strain was present. In flooded microcosms with the USDA110(T)wt strain, USDA110(T)wt became dominant, whereas in microcosms with the USDA110 Delta nosZ, a similar change in the community structure occurred to that in non-flooded microcosms. These results suggest that possession of the nosZ gene confers a competitive advantage to B. diazoefficiens USDA110(T) in flooded soil. We herein demonstrated that the dominance of B. diazoefficiens USDA110(T)wt within the soil bradyrhizobial population may be enhanced by periods of flooding or waterlogging systems such as paddy-soybean rotations because it appears to have the ability to thrive in moderately anaerobic soil.

リンク情報
DOI
https://doi.org/10.1264/jsme2.ME16132
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000404480300009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1264/jsme2.ME16132
  • ISSN : 1342-6311
  • Web of Science ID : WOS:000404480300009

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