Microfluidic cell culturing in channels is required to deliver a cell suspension from an external source to the channels where the cells are attached to the bottom surface of it. But cell types, which require frequent feeding, are difficult to attach to the substrate of micro-fluidic channels in static culture medium, because the cells deplete nutrient and oxygen until sufficient cell attachment. We present for a new micro-fluidic culturing method which can perfuse medium m the channels without flushing cells which deposit on the substrate in gravity. Our approach eliminates the need to hold cells by micro-structures in perfusion cultivation.