MISC

2010年9月

Exercise training attenuates anaphylactic venoconstriction in rat perfused liver, but does not affect anaphylactic hypotension in conscious rats

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
  • Sen Cui
  • ,
  • Toshishige Shibamoto
  • ,
  • Wei Zhang
  • ,
  • Yasutaka Kurata
  • ,
  • Osamu Kashimura
  • ,
  • Shunichi Miyamae

37
9
開始ページ
889
終了ページ
893
記述言語
英語
掲載種別
DOI
10.1111/j.1440-1681.2010.05401.x
出版者・発行元
WILEY-BLACKWELL

P>1. Exercise training attenuates circulatory shock due to haemorrhage, endotoxin or heatstroke. However, it remains unknown whether exercise training attenuates anaphylactic shock. Hepatic venoconstriction is involved in rat anaphylactic hypotension. In the present study, we determined the effects of exercise training on both anaphylaxis-induced segmental venoconstriction in rat perfused livers and systemic anaphylaxis in conscious rats. The role of nitric oxide (NO) in the effect of exercise on the venoconstriction of perfused livers was also examined.
2. Rats were subjected to running training on a motorized treadmill for 4 weeks. Two weeks prior to the anaphylaxis experiment, Sprague-Dawley rats were actively sensitized with the antigen ovalbumin. In isolated livers perfused portally with blood, the portal venous pressure (P(pv)) and sinusoidal pressure were measured to determine the pre- and post-sinusoidal resistances (R(pre) and R(post), respectively). In conscious rats, systemic arterial pressure (SAP) and P(pv) were determined.
3. In the perfused livers of sedentary rats, antigen administration led to a predominant presinusoidal constriction, as evidenced by 4.6- and 1.7-fold increases in R(pre) and R(post), respectively. The anaphylaxis-induced increase in R(pre) was significantly attenuated by 24% by exercise training. Inhibition of NO synthase with NG-nitro-l-arginine methyl ester (100 mu mol/L) 10 min prior to the injection of antigen enhanced anaphylactic venoconstriction, but did not alter the effect of exercise training on the increase in R(pre). In contrast, exercise training did not attenuate either anaphylactic hypotension or portal hypertension in conscious rats.
4. In conclusion, exercise training attenuates the anaphylaxis-induced presinusoidal constriction in rat isolated perfused livers, independent of NO production. However, this action is not evident in conscious rats and exercise training does not affect anaphylactic hypotension in conscious rats.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1111/j.1440-1681.2010.05401.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20456421
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000281219600005&DestApp=WOS_CPL

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