MISC

2001年9月

Preoviposition activation of cathepsin-like proteinases in degenerating ovarian follicles of the mosquito Culex pipiens pallens

DEVELOPMENTAL BIOLOGY
  • K Uchida
  • ,
  • D Ohmori
  • ,
  • T Ueno
  • ,
  • M Nishizuka
  • ,
  • Y Eshita
  • ,
  • A Fukunaga
  • ,
  • E Kominami

237
1
開始ページ
68
終了ページ
78
記述言語
英語
掲載種別
DOI
10.1006/dbio.2001.0357
出版者・発行元
ACADEMIC PRESS INC

Within developing ovaries of many insects, some developing follicles or oocytes usually degenerate (follicular atresia or oosorption), while the others may continue to grow to maturity, thus maintaining the balance between the number of eggs and reproductive circumstances such as available nutrients. To help clarify the phenomenon of follicular atresia during ovarian development, we examined cysteine proteinases stored in mosquito Culex pipiens pollens ovaries. First, analysis using synthesized substrates showed that cathepsin B- and L-like proteinases gradually accumulated in the developing ovaries after a blood meal, which required more than 10 min of preincubation under acidic conditions to reach their maximum activities. However, homogenates of degenerating follicles 3 days after feeding showed proteolytic activities without acid treatment, suggesting that the proteinases had already been activated, while the extract of normally developing follicles collected from the same ovaries required more than 10 min of acid preincubation to reach the optimum activities, suggesting that the enzymes remained as inactive forms. Chemical and immunohistochemical analyses showed that more proteinases are located in the cytoplasm, rather than being associated with yolk granules. Ovarian proteinases, which are believed to become activated at the onset of embryogenesis, should also be activated during oogenesis, presumably to enhance oosorption. (C) 2001 Academic Press.

Web of Science ® 被引用回数 : 39

リンク情報
DOI
https://doi.org/10.1006/dbio.2001.0357
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000170882100006&DestApp=WOS_CPL

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