論文

査読有り
2005年12月

Microscopic imaging of phase separation in giant liposome by using laurdan at video rate

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS
  • T Ohba
  • ,
  • S Tsuchiya
  • ,
  • T Kumeta
  • ,
  • K Ohki

44
12
開始ページ
8733
終了ページ
8738
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1143/JJAP.44.8733
出版者・発行元
JAPAN SOC APPLIED PHYSICS

Laurdan is a useful fluorescence dye for observation of physical properties of membranes. An optical system was constructed in order to image generalized polarization (GP), that characterizes the change of the spectrum of laurdan. The fluorescence images through the optical system were recorded on a digital video recorder and the images were processed to GP images, which made it possible to measure the spatial distribution of membrane fluidity quantitatively at video rate. Using this imaging instrument, the change of membrane fluidity at every moment of phase transition was observed in a giant liposome of pure dimyristoylphosphatidylcholine and the dynamics of phase separation was observed in a giant liposome composed of dimyristoylphosphatidylcholine and dimyristoylphosphatidylethaiiolaiiiine after an annealing process. A clear phase separation was observed in the giant liposome in the cooling process from 43 to 40 degrees C. The GP value of the phase-separated region on the liposome was related to the shape of the same region. The linear part of the liposome had a high GP value and the hinge part had a low GP value.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1143/JJAP.44.8733
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000234223800088&DestApp=WOS_CPL
ID情報
  • DOI : 10.1143/JJAP.44.8733
  • ISSN : 0021-4922
  • Web of Science ID : WOS:000234223800088

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