2013年
The deamination of APOBEC3G os controlled by the sliding-direction-dependent catalytic activity, and anti-prion activity of an RNA aptamer and its structural basis
The 10th Korea-Japan Bilateral Symposium
- 巻
- 41
- 号
- 2
- 開始ページ
- 1355
- 終了ページ
- 1362
- 記述言語
- 英語
- 掲載種別
- 研究発表ペーパー・要旨(全国大会,その他学術会議)
- DOI
- 10.1093/nar/gks1132
- 出版者・発行元
- Oxford University Press
Prion proteins (PrPs) cause prion diseases, such as bovine spongiform encephalopathy. The conversion of a normal cellular form (PrP(C)) of PrP into an abnormal form (PrP(Sc)) is thought to be associated with the pathogenesis. An RNA aptamer that tightly binds to and stabilizes PrP(C) is expected to block this conversion and to thereby prevent prion diseases. Here, we show that an RNA aptamer comprising only 12 residues, r(GGAGGAGGAGGA) (R12), reduces the PrP(Sc) level in mouse neuronal cells persistently infected with the transmissible spongiform encephalopathy agent. Nuclear magnetic resonance analysis revealed that R12, folded into a unique quadruplex structure, forms a dimer and that each monomer simultaneously binds to two portions of the N-terminal half of PrP(C), resulting in tight binding. Electrostatic and stacking interactions contribute to the affinity of each portion. Our results demonstrate the therapeutic potential of an RNA aptamer as to prion diseases.
- リンク情報
- ID情報
-
- DOI : 10.1093/nar/gks1132
- ISSN : 1362-4962
- CiNii Articles ID : 120005244396