2015年10月6日
H-ferritin is preferentially incorporated by human erythroid cells through transferrin receptor 1 in a threshold-dependent manner
PLoS ONE
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- 巻
- 10
- 号
- 10
- 開始ページ
- e0139915.
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0139915
- 出版者・発行元
- Public Library of Science
Ferritin is an iron-storage protein composed of different ratios of 24 light (L) and heavy (H) subunits. The serum level of ferritin is a clinical marker of the body's iron level. Transferrin receptor (TFR)1 is the receptor not only for transferrin but also for H-ferritin, but how it binds two different ligands and the blood cell types that preferentially incorporate H-ferritin remain unknown. To address these questions, we investigated hematopoietic cell-specific ferritin uptake by flow cytometry. Alexa Fluor 488-labeled H-ferritin was preferentially incorporated by erythroid cells among various hematopoietic cell lines examined, and was almost exclusively incorporated by bone marrow erythroblasts among human primary hematopoietic cells of various lineages. H-ferritin uptake by erythroid cells was strongly inhibited by unlabeled H-ferritin but was only partially inhibited by a large excess of holo-transferrin. On the other hand, internalization of labeled holo-transferrin by these cells was not inhibited by H-ferritin. Chinese hamster ovary cells lacking functional endogenous TFR1 but expressing human TFR1 with a mutated RGD sequence, which is required for transferrin binding, efficiently incorporated H-ferritin, indicating that TFR1 has distinct binding sites for H-ferritin and holo-transferrin. H-ferritin uptake by these cells required a threshold level of cell surface TFR1 expression, whereas there was no threshold for holo-transferrin uptake. The requirement for a threshold level of TFR1 expression can explain why among primary human hematopoietic cells, only erythroblasts efficiently take up H-ferritin.
- リンク情報
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- DOI
- https://doi.org/10.1371/journal.pone.0139915
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/26441243
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84947999017&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84947999017&origin=inward
- ID情報
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- DOI : 10.1371/journal.pone.0139915
- ISSN : 1932-6203
- eISSN : 1932-6203
- PubMed ID : 26441243
- SCOPUS ID : 84947999017