1996年9月
Serial transplantation of adult T cell leukemia cells into severe combined immunodeficient mice
JAPANESE JOURNAL OF CANCER RESEARCH
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- 巻
- 87
- 号
- 9
- 開始ページ
- 887
- 終了ページ
- 892
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- JAPANESE CANCER ASSOCIATION
The precise mechanism of the neoplastic cell growth of adult T cell leukemia (ATL) still remains unclear. In the present study, we have succeeded in serial transplantation of ATL cells from a patient into severe combined immunodeficient (SCID) mice. In this model, we found that only a leukemic cell clone from an ATL patient could be successively transplanted into SCID mice, although it was difficult to maintain leukemic cell clones in vitro, suggesting that the microenvironment provided by SCID mice is suitable for leukemic cell growth. We could not detect human T cell leukemia virus type I (HTLV-I) mRNA or interleukin 2 (IL-2) mRNA in either the tumor cells growing in mice or the original leukemic cells. Thus, it appears that neither HTLV-I viral expression nor the IL-2 autocrine mechanism is directly involved in the neoplastic cell growth of fresh ATL cells as well as HTLV-I-infected cell lines, at least in SCID mice. In addition, we could passage frozen cells and obtain a large number of expanded leukemic cells in this model. Such a serial transplantation model, which can avoid the changes in the nature of leukemic cells that are frequently observed in in vitro culture, and which can propagate leukemic cell clones, would be very suitable not only to study the mechanism of neoplastic cell growth, but also to test potential therapeutic agents for ATL.
- リンク情報
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- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902152427777649
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/8878449
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1996VL78900004&DestApp=WOS_CPL
- ID情報
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- ISSN : 0910-5050
- J-Global ID : 200902152427777649
- PubMed ID : 8878449
- Web of Science ID : WOS:A1996VL78900004