論文

2022年3月20日

Assessment of Ultra-Early-Stage Liver Fibrosis in Human Non-Alcoholic Fatty Liver Disease by Second-Harmonic Generation Microscopy

International Journal of Molecular Sciences
  • Takeo Minamikawa
  • ,
  • Eiji Hase
  • ,
  • Mayuko Ichimura-Shimizu
  • ,
  • Yuki Morimoto
  • ,
  • Akihiro Suzuki
  • ,
  • Takeshi Yasui
  • ,
  • Satoko Nakamura
  • ,
  • Akemi Tsutsui
  • ,
  • Koichi Takaguchi
  • ,
  • Koichi Tsuneyama

23
6
開始ページ
3357
終了ページ
3357
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/ijms23063357
出版者・発行元
{MDPI} {AG}

Non-alcoholic fatty liver disease (NAFLD) is associated with the chronic progression of fibrosis. In general, the progression of liver fibrosis is determined by a histopathological assessment with a collagen-stained section; however, the ultra-early stage of liver fibrosis is challenging to identify because of the low sensitivity in the collagen-selective staining method. In the present study, we demonstrate the feasibility of second-harmonic generation (SHG) microscopy in the histopathological diagnosis of the liver of NAFLD patients for the quantitative assessment of the ultra-early stage of fibrosis. We investigated four representative NAFLD patients with early stages of fibrosis. SHG microscopy visualised well-matured fibrotic structures and early fibrosis diffusely involving liver tissues, whereas early fibrosis is challenging to be identified by conventional histopathological methods. Furthermore, the SHG emission directionality analysis revealed the maturation of each collagen fibre of each patient. As a result, SHG microscopy is feasible for assessing liver fibrosis on NAFLD patients, including the ultra-early stage of liver fibrosis that is difficult to diagnose by the conventional histopathological method. The assessment method of the ultra-early fibrosis by using SHG microscopy may serve as a crucial means for pathological, clinical, and prognostic diagnosis of NAFLD patients.

リンク情報
DOI
https://doi.org/10.3390/ijms23063357
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85126575411&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85126575411&origin=inward
ID情報
  • DOI : 10.3390/ijms23063357
  • ISSN : 1661-6596
  • eISSN : 1422-0067
  • ORCIDのPut Code : 110524984
  • SCOPUS ID : 85126575411

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