論文

査読有り 筆頭著者 責任著者 本文へのリンクあり 国際誌
2019年5月16日

Tracking and clarifying differential traits of classical- and atypical L-type bovine spongiform encephalopathy prions after transmission from cattle to cynomolgus monkeys.

PLOS ONE
  • Ken’ichi Hagiwara
  • ,
  • Yuko Sato
  • ,
  • Yoshio Yamakawa
  • ,
  • Hideyuki Hara
  • ,
  • Minoru Tobiume
  • ,
  • Yuko Okemoto-Nakamura
  • ,
  • Tetsutaro Sata
  • ,
  • Motohiro Horiuchi
  • ,
  • Hiroaki Shibata
  • ,
  • Fumiko Ono

14
5
開始ページ
e0216807
終了ページ
e0216807
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0216807
出版者・発行元
Public Library of Science (PLoS)

Classical- (C-) and atypical L-type bovine spongiform encephalopathy (BSE) prions cause different pathological phenotypes in cattle brains, and the disease-associated forms of each prion protein (PrPSc) has a dissimilar biochemical signature. Bovine C-BSE prions are the causative agent of variant Creutzfeldt-Jakob disease. To date, human infection with L-BSE prions has not been reported, but they can be transmitted experimentally from cows to cynomolgus monkeys (Macaca fascicularis), a non-human primate model. When transmitted to monkeys, C- and L-BSE prions induce different pathological phenotypes in the brain. However, when isolated from infected brains, the two prion proteins (PrPSc) have similar biochemical signatures (i.e., electrophoretic mobility, glycoforms, and resistance to proteinase K). Such similarities suggest the possibility that L-BSE prions alter their virulence to that of C-BSE prions during propagation in monkeys. To clarify this possibility, we conducted bioassays using inbred mice. C-BSE prions with or without propagation in monkeys were pathogenic to mice, and exhibited comparable incubation periods in secondary passage in mice. By contrast, L-BSE prions, either with or without propagation in monkeys, did not cause the disease in mice, indicating that the pathogenicity of L-BSE prions does not converge towards a C-BSE prion type in this primate model. These results suggest that, although C- and L-BSE prions propagated in cynomolgus monkeys exhibit similar biochemical PrPSc signatures and consist of the monkey amino acid sequence, the two prions maintain strain-specific conformations of PrPSc in which they encipher and retain unique pathogenic traits.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0216807 本文へのリンクあり
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31095605
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522098
URL
http://dx.plos.org/10.1371/journal.pone.0216807
ID情報
  • DOI : 10.1371/journal.pone.0216807
  • ISSN : 1932-6203
  • eISSN : 1932-6203
  • PubMed ID : 31095605
  • PubMed Central 記事ID : PMC6522098

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