2014年
A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
- 巻
- 60
- 号
- 1
- 開始ページ
- 44
- 終了ページ
- 50
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.2323/jgam.60.44
- 出版者・発行元
- MICROBIOL RES FOUNDATION
Small regulatory RNAs (sRNAs) are conserved among a wide range of bacteria. They modulate the translational efficiency of target mRNAs through base-pairing with the help of RNA chaperone Hfq. The present study identified a novel sRNA, Esr41 (enterohemorrhagic Escherichia coli O157 small RNA #41), from an intergenic region of an enterohemorrhagic E. coli (EHEC) O157:H7 Sakai-specific sequence that is not present in the nonpathogenic E. coli K-12. Esr41 was detected as an RNA molecule approximately 70 nucleotides long with a 3' GC-rich palindrome sequence followed by a long poly(U), which is a characteristic of rho-independent terminators and is also a structural feature required for the action of Hfq. EHEC O157 harboring a multicopy plasmid carrying the esr41 gene increased cell motility and the expression of fliC, a gene encoding a major flagellar component. These results indicate that Esr41 stimulates fliC expression in EHEC O157. Furthermore, the increase in cell motility induced by Esr41 was also observed in the E. coli K-12, suggesting that target genes controlled by Esr41 are present in both EHEC O157 and K-12.
- リンク情報
- ID情報
-
- DOI : 10.2323/jgam.60.44
- ISSN : 0022-1260
- eISSN : 1349-8037
- Web of Science ID : WOS:000333019500008