We have fabricated a flexible hydroxyapatite (HAp) sheet using pulsed laser deposition and a sheet isolation technique. We aim to form an artificial enamel layer on the dentin by an amorphous calcium phosphate (ACP) sheet with solubility higher than compared that of the crystallized sheet. In this study, using Pashle's method, we evaluated of the dentin permeability inhibition effect by double layer method of the ACP sheet on dentin as a cure for dental hypersensitivity. The dentin permeability inhibition rate of the dentinal tubule by double layer method of the ACP sheet was 89.17±7.24%. The ACP sheet was yielded results better than of dental resin cement.