1994
INDUCTION OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) AND GRANULOCYTE-COLONY-STIMULATING FACTOR (G-CSF) EXPRESSION IN BONE-MARROW AND FRACTIONATED MARROW CELL-POPULATIONS BY INTERLEUKIN-3 (IL-3) - IL-3-MEDIATED POSITIVE FEEDBACK MECHANISMS OF GRANULOPOIESIS
GROWTH FACTORS
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- Volume
- 11
- Number
- 1
- First page
- 71
- Last page
- 79
- Language
- English
- Publishing type
- Research paper (scientific journal)
- Publisher
- HARWOOD ACAD PUBL GMBH
Interleukin 3 (IL-3) induces proliferation and differentiation of mast cell progenitors in vitro, whereas it induces granulocytosis in vivo. In this paper, a positive feedback mechanism of granulopoiesis was studied in order to elucidate the granulocytosis induced by IL-3 in mouse. IL-3 induced expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) in total bone marrow cells and a marrow adherent cell population. In fractionated marrow cell populations, a different expression pattern of induction by IL-3 stimulation was observed; GM-CSF was expressed in macrophages and fraction 1 (P<1.061) and 2(1.061<P<1.074) of bone marrow cells fractionated by equilibrium density centrifugation, G-CSF was expressed in macrophages and fraction 2 and 3 (1.074<P<1.097), and interleukin 6 (IL-6) in macrophages and fraction 1 to 3. These results indicate a hierarchical regulation of cytokine production and the existence of a positive feedback mechanism in granulopoiesis. IL-6, induced by IL-3, stimulates stem cells into cycle and induces stem cells to respond to IL-3. The stem cells differentiate to granulocyte-macrophage colony-forming cells by the combined effect of IL-3 and IL-6. IL-3 also induces GM- and G-CSF expression which in turn makes granulocyte-macrophage colony-forming cells differentiate to granulocytes. These factors organize a cytokine network in granulopoiesis.
- Link information
- ID information
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- ISSN : 0897-7194
- Web of Science ID : WOS:A1994PL13500007