論文

査読有り
2002年4月

Identification of negative regulator of interleukin-3 (NIL-3) in bone marrow.

Cell structure and function
  • Adachi Yasuhiro
  • ,
  • Kenkichi Sugimoto
  • ,
  • Asako K Sato
  • ,
  • Kazuhiro John Mori

27
2
開始ページ
81
終了ページ
9
記述言語
英語
掲載種別
研究論文(学術雑誌)

We have reported that an inhibitor of interleukin-3 (NIL-3) is produced from murine bone marrow cells in response to excess stimulation of interleukin-3. In this report, we attempted the purification of the NIL-3 activity from bone marrow culture supernatant in the presence of interleukin-3. The purified NIL-3 activity was a protein with relative molecular weight of 54.5 kDa (SDS-PAGE), which inhibited the growth of IL-3 dependent DA-1 cell growth in a dose dependent manner. The N-terminal amino acid sequence of purified NIL-3 activity was determined to be homologous to beta-2 glycoprotein I (apolipoprotein H: APO-H). The gene expression of APO-H was detected by nested-PCR in STIL-3 C5-CM stimulated total bone marrow cells and STIL-3 C5-CM stimulated bone marrow fraction 2 (Fr. 2) which has been reported as a hematopoietic stem cell rich fraction. These observations indicate the possibility that the APO-H is the NIL-3 which was produced from bone marrow cells in response to excess IL-3 stimuli.

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12207049
ID情報
  • ISSN : 0386-7196
  • PubMed ID : 12207049

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