<sec>
<title>Background:</title>
Sargassum polycystum C. Agardh has potent antioxidant and anti-inflammatory properties.
However, its anti-allergic effect has not yet been reported. In this study, we investigated the anti-allergic effects of sulfated
polysaccharide of S. polycystum (SPSP) in Dinitrofluorobenzene (DNFB)- induced allergic contact dermatitis animal model.


</sec>
<sec>
<title>Methods:</title>
SPSP was extracted through hot water extraction method and was subjected to compositional analyses. For the
allergic contact dermatitis (ACD) model, symptoms were induced by the topical application of 0.5% DNFB on the shaved
ventral skin of mice. SPSP (500, 1000, and 2000 mg/kg) and Prednisolone were orally administered for seven days after
sensitization. Elicitation was performed seven days later with 0.2% DNFB. After this, ear thickness was measured at baseline
and 24 hours post elicitation using a dial thickness gauge. Serum of mice was obtained 24 hours post elicitation, and the
level of IFNγ and TNF


</sec>
<sec>
<title>Results:</title>
SPSP afforded 33.6% carbohydrates, 23.7% sulfate, 7.5% protein, and 1.5% uronic acid contents. SPSP inhibited
the ear swelling and cytokines (IFNγ and TNF


</sec>
<sec>
<title>Conclusion:</title>
These findings showed that the sulfated polysaccharide from S. polycytum is a potential natural source to treat
Allergic Contact Dermatitis. The effect is attributed to polysaccharide-protein complex present in the extract, but further
studies are needed to establish the exact mechanism of action of SPSP in the treatment of the disease.



</sec>