2017年1月
Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea
SCIENTIFIC REPORTS
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- 巻
- 7
- 号
- 開始ページ
- 40368
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep40368
- 出版者・発行元
- NATURE PUBLISHING GROUP
Environmental DNA (eDNA) metabarcoding has emerged as a potentially powerful tool to assess aquatic community structures. However, the method has hitherto lacked field tests that evaluate its effectiveness and practical properties as a biodiversity monitoring tool. Here, we evaluated the ability of eDNA metabarcoding to reveal fish community structures in species-rich coastal waters. High-performance fish-universal primers and systematic spatial water sampling at 47 stations covering similar to 11 km(2) revealed the fish community structure at a species resolution. The eDNA metabarcoding based on a 6-h collection of water samples detected 128 fish species, of which 62.5% (40 species) were also observed by underwater visual censuses conducted over a 14-year period. This method also detected other local fishes (>= 23 species) that were not observed by the visual censuses. These eDNA metabarcoding features will enhance marine ecosystem-related research, and the method will potentially become a standard tool for surveying fish communities.
- リンク情報
- ID情報
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- DOI : 10.1038/srep40368
- ISSN : 2045-2322
- Web of Science ID : WOS:000391675300002