論文

査読有り 国際誌
2021年4月20日

Development of Anti-Mouse CC Chemokine Receptor 3 Monoclonal Antibodies for Flow Cytometry.

Monoclonal antibodies in immunodiagnosis and immunotherapy
  • Teizo Asano
  • ,
  • Ren Nanamiya
  • ,
  • Junko Takei
  • ,
  • Takuro Nakamura
  • ,
  • Miyuki Yanaka
  • ,
  • Hideki Hosono
  • ,
  • Tomohiro Tanaka
  • ,
  • Masato Sano
  • ,
  • Mika K Kaneko
  • ,
  • Yukinari Kato

記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/mab.2021.0009

CC chemokine receptor 3 (CCR3), also known as CD193, belongs to class A of G protein-coupled receptors and is present in high levels in eosinophils, basophils, and airway epithelial cells. CCR3 is considered the therapeutic target for human immunodeficiency virus (HIV) infections and allergic diseases; therefore, the development of sensitive monoclonal antibodies (mAbs) for CCR3 has been desired. This study aimed to establish a specific and sensitive mAb against mouse CCR3 (mCCR3) useful for flow cytometry analysis by employing the Cell-Based Immunization and Screening (CBIS) method. The generated anti-mCCR3 mAb, C3Mab-2 (rat IgG2b, kappa), was found to react with mCCR3-overexpressed Chinese hamster ovary-K1 (CHO/mCCR3) cells, according to flow cytometric analysis. Also, it reacted with P388 (mouse lymphoid neoplasm) or J774-1 (mouse macrophage-like) cells, which express endogenous mCCR3. Taken together, C3Mab-2, generated by the CBIS method, can be a valuable tool for detecting mCCR3 on the surface of mouse cells.

リンク情報
DOI
https://doi.org/10.1089/mab.2021.0009
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33877898
ID情報
  • DOI : 10.1089/mab.2021.0009
  • PubMed ID : 33877898

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