論文

査読有り 国際誌
2021年4月

Development of Anti-Mouse CC Chemokine Receptor 8 Monoclonal Antibodies for Flow Cytometry.

Monoclonal antibodies in immunodiagnosis and immunotherapy
  • Tomohiro Tanaka
  • ,
  • Ren Nanamiya
  • ,
  • Junko Takei
  • ,
  • Takuro Nakamura
  • ,
  • Miyuki Yanaka
  • ,
  • Hideki Hosono
  • ,
  • Masato Sano
  • ,
  • Teizo Asano
  • ,
  • Mika K Kaneko
  • ,
  • Yukinari Kato

40
2
開始ページ
65
終了ページ
70
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/mab.2021.0005

CC chemokine receptor 8 (CCR8) belongs to the class A of G protein-coupled receptor. It is highly expressed on Treg and T helper 2 (TH2) cells recruited to the inflammation site and is implicated in allergy and asthma. Recently, CCR8+Treg cells have been suggested to be a master regulator in the immunosuppressive tumor microenvironment; therefore, developing sensitive monoclonal antibodies (mAbs) for CCR8 has been desired. This study established a specific and sensitive mAb for mouse CCR8 (mCCR8), which is useful for flow cytometry by using the Cell-Based Immunization and Screening (CBIS) method. The established anti-mCCR8 mAb, C8Mab-2 (rat IgG2b, kappa), reacted with mCCR8-overexpressed Chinese hamster ovary-K1 (CHO/mCCR8) cells and P388 (mouse lymphoid neoplasma) or J774-1 (mouse macrophage-like) cells, which express endogenous mCCR8 by flow cytometry. C8Mab-2, which was established by the CBIS method, could be useful for elucidating the mCCR8-related biological response by flow cytometry.

リンク情報
DOI
https://doi.org/10.1089/mab.2021.0005
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33900818
ID情報
  • DOI : 10.1089/mab.2021.0005
  • PubMed ID : 33900818

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