論文

査読有り 国際誌
2021年6月

Ferret Podoplanin Is Detected by PMab-241 in Immunohistochemistry.

Monoclonal antibodies in immunodiagnosis and immunotherapy
  • Ren Nanamiya
  • ,
  • Junko Takei
  • ,
  • Teizo Asano
  • ,
  • Masato Sano
  • ,
  • Tomohiro Tanaka
  • ,
  • Hideki Hosono
  • ,
  • Hiroyuki Harada
  • ,
  • Yusuke Sakai
  • ,
  • Takuya Mizuno
  • ,
  • Hiroyoshi Suzuki
  • ,
  • Mika K Kaneko
  • ,
  • Yukinari Kato

40
3
開始ページ
134
終了ページ
140
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/mab.2021.0016

Podoplanin (PDPN) plays an important role in the development of many normal tissues and is expressed in various cancers. We have previously developed multiple monoclonal antibodies (mAbs) against PDPNs from a variety of animal species and characterized each of these PDPNs using the anti-PDPN mAbs. In this study, we evaluated whether these anti-PDPN mAbs possess cross-reactivity with ferret PDPN (ferPDPN) using flow cytometry. Comprehensive analysis using 17 differing anti-PDPN mAbs available for immunohistochemistry use, demonstrated that the anti-bear PDPN mAb (clone PMab-241) strongly cross-reacts with ferPDPN-overexpressed Chinese hamster ovary-K1 (CHO/ferPDPN) cells. Immunohistochemistry analysis demonstrated intense PMab-241 staining within Bowman's capsules and glomeruli of the ferret kidney, and lymphatic endothelial cells of the ferret lung. These results demonstrate that PMab-241 is suitable for the detection of PDPN in ferret tissues.

リンク情報
DOI
https://doi.org/10.1089/mab.2021.0016
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34161160
ID情報
  • DOI : 10.1089/mab.2021.0016
  • PubMed ID : 34161160

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