論文

査読有り 国際誌
2021年8月

Epitope Mapping of the Anti-California Sea Lion Podoplanin Monoclonal Antibody PMab-269 Using Alanine-Scanning Mutagenesis and ELISA.

Monoclonal antibodies in immunodiagnosis and immunotherapy
  • Tomohiro Tanaka
  • ,
  • Teizo Asano
  • ,
  • Masato Sano
  • ,
  • Junko Takei
  • ,
  • Hideki Hosono
  • ,
  • Ren Nanamiya
  • ,
  • Nami Tateyama
  • ,
  • Mika K Kaneko
  • ,
  • Yukinari Kato

40
4
開始ページ
196
終了ページ
200
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/mab.2021.0017

Podoplanin (PDPN) plays a pivotal role in platelet aggregation, embryo development, and tumor progression. PDPN is universally expressed in many mammalian species, and is considered a typical lymphatic endothelial cell marker. We have previously developed the mouse anti-California sea lion (Zalophus californianus) PDPN (seaPDPN) monoclonal antibody (mAb), clone PMab-269, which is suitable for different experimental applications, including flow cytometry, Western blotting, and immunohistochemistry. In this study, we identified the PMab-269 epitope of the seaPDPN by enzyme-linked immunosorbent assay using deletion mutants and point mutants generated for seaPDPN. Our results demonstrated that PMab-269 recognized the peptide, corresponding to the amino acids 63-82 of seaPDPN. Furthermore, the reactions of PMab-269 to seven alanine-substituted peptides, such as P68A, D76A, F77A, H78A, L79A, E80A, and D81A, were abolished among 20 alanine-substituted peptides. We identified the seven amino acids (Pro68, Asp76, Phe77, His78, Leu79, Glu80, and Asp81) as the critical epitope targeted by PMab-269. The successful identification of the PMab-269 epitope might contribute to the pathophysiological investigations of seaPDPN.

リンク情報
DOI
https://doi.org/10.1089/mab.2021.0017
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34283661
ID情報
  • DOI : 10.1089/mab.2021.0017
  • PubMed ID : 34283661

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