論文

査読有り 国際誌
2020年7月4日

RNA sequencing-based microRNA expression signature in esophageal squamous cell carcinoma: oncogenic targets by antitumor miR-143-5p and miR-143-3p regulation.

Journal of human genetics
  • Masumi Wada
  • Yusuke Goto
  • Takako Tanaka
  • Reona Okada
  • Shogo Moriya
  • Tetsuya Idichi
  • Masahiro Noda
  • Ken Sasaki
  • Yoshiaki Kita
  • Hiroshi Kurahara
  • Kosei Maemura
  • Shoji Natsugoe
  • Naohiko Seki
  • 全て表示

65
11
開始ページ
1019
終了ページ
1034
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s10038-020-0795-x

Aberrantly expressed microRNAs (miRNAs) disrupt intracellular RNA networks and contribute to malignant transformation of cancer cells. Utilizing the latest RNA sequencing technology, we newly created the miRNA expression signature of esophageal squamous cell carcinoma (ESCC). A total of 47 miRNAs were downregulated in ESCC tissues, and these miRNAs were candidates for antitumor miRNAs in ESCC cells. Analysis of the signature revealed that several passenger strands of miRNAs were significantly downregulated in ESCC, e.g., miR-28-3p, miR-30a-3p, miR-30c-3p, miR-133a-3p, miR-139-3p, miR-143-5p, and miR-145-3p. Recent studies indicate that some passenger strands of miRNAs closely involved in cancer pathogenesis. In this study, we focused on both strands of pre-miR-143, and investigated their antitumor roles and target oncogenes in ESCC. Ectopic expression of miR-143-5p and miR-143-3p significantly attenuated malignant phenotypes (e.g., proliferation, migration, and invasive abilities) in ESCC cell lines. We revealed that six genes (HN1, HMGA2, NETO2, STMN1, TCF3, and MET) were putative targets of miR-143-5p regulation, and one gene (KRT80) was a putative target of miR-143-3p regulation in ESCC cells. Our ESCC miRNA signature and analysis strategy provided important insights into the molecular pathogenesis of ESCC.

リンク情報
DOI
https://doi.org/10.1038/s10038-020-0795-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32623445
ID情報
  • DOI : 10.1038/s10038-020-0795-x
  • PubMed ID : 32623445

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