論文

査読有り 国際誌
2019年4月

Aggregatilinea lenta gen. nov., sp. nov., a slow-growing, facultatively anaerobic bacterium isolated from subseafloor sediment, and proposal of the new order Aggregatilineales ord. nov. within the class Anaerolineae of the phylum Chloroflexi.

International journal of systematic and evolutionary microbiology
  • Nozomi Nakahara
  • ,
  • Masaru K Nobu
  • ,
  • Yoshihiro Takaki
  • ,
  • Masayuki Miyazaki
  • ,
  • Eiji Tasumi
  • ,
  • Sanae Sakai
  • ,
  • Miyuki Ogawara
  • ,
  • Naoko Yoshida
  • ,
  • Hideyuki Tamaki
  • ,
  • Yuko Yamanaka
  • ,
  • Arata Katayama
  • ,
  • Takashi Yamaguchi
  • ,
  • Ken Takai
  • ,
  • Hiroyuki Imachi

69
4
開始ページ
1185
終了ページ
1194
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1099/ijsem.0.003291

A novel slow-growing, facultatively anaerobic, filamentous bacterium, strain MO-CFX2T, was isolated from a methanogenic microbial community in a continuous-flow bioreactor that was established from subseafloor sediment collected off the Shimokita Peninsula of Japan. Cells were multicellular filamentous, non-motile and Gram-stain-negative. The filaments were generally more than 20 µm (up to approximately 200 µm) long and 0.5-0.6 µm wide. Cells possessed pili-like structures on the cell surface and a multilayer structure in the cytoplasm. Growth of the strain was observed at 20-37 °C (optimum, 30 °C), pH 5.5-8.0 (pH 6.5-7.0), and 0-30 g l-1 NaCl (5 g l-1 NaCl). Under optimum growth conditions, doubling time and maximum cell density were estimated to be approximately 19 days and ~105 cells ml-1, respectively. Strain MO-CFX2T grew chemoorganotrophically on a limited range of organic substrates in anaerobic conditions. The major cellular fatty acids were saturated C16 : 0 (47.9 %) and C18 : 0 (36.9 %), and unsaturated C18 : 1ω9c (6.0 %) and C16 : 1ω7 (5.1 %). The G+C content of genomic DNA was 63.2 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain MO-CFX2T shares a notably low sequence identity with its closest relatives, which were Thermanaerothrix daxensis GNS-1T and Thermomarinilinea lacunifontana SW7T (both 85.8 % sequence identity). Based on these phenotypic and genomic properties, we propose the name Aggregatilinea lenta gen. nov., sp. nov. for strain MO-CFX2T (=KCTC 15625T, =JCM 32065T). In addition, we also propose the associated family and order as Aggregatilineaceae fam. nov. and Aggregatilineales ord. nov., respectively.

リンク情報
DOI
https://doi.org/10.1099/ijsem.0.003291
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30775966
URL
http://orcid.org/0000-0001-5426-814X
ID情報
  • DOI : 10.1099/ijsem.0.003291
  • ISSN : 1466-5034
  • ISSN : 1466-5026
  • ORCIDのPut Code : 56476475
  • PubMed ID : 30775966

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