2017年2月
A rapid and precise method for measuring plasma apoE-rich HDL using polyethylene glycol and cation-exchange chromatography: a pilot study on the clinical significance of apoE-rich HDL measurements
CLINICA CHIMICA ACTA
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- 巻
- 465
- 号
- 開始ページ
- 112
- 終了ページ
- 118
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.cca.2016.12.016
- 出版者・発行元
- ELSEVIER SCIENCE BV
Background: High-density lipoprotein (HDL) containing apolipoprotein E (apoE-rich HDL) represents only a small portion of plasma HDL. Reliable methods for determining and isolating apoE-rich HDL have not been well studied.
Methods: We established a novel analytical method for apoE-rich HDL using polyethylene glycol and a cation-exchange column (PEG-column method). Furthermore, we examined biochemical correlates of apoE-rich HDL-cholesterol (HDL-C) in 36 patients who underwent coronary computed tomographic angiography.
Results: Our PEG-column method demonstrated high reproducibility (coefficient of variation <3.52%) and linearity up to 15 mg/dl for apoE-rich HDL-C concentrations. Isolated apoE-rich HDL exhibited a larger diameter (14.8 nm) than apoE-poor HDL (10.8 nm) and contained both apoE and apoA-I. ApoE-rich HDL-C concentrations correlated significantly with triglycerides (r(s) = -0.646), LDL size (r(s) = 0.472), adiponectin (r(s) = 0.476), and other lipoprotein components. No significant correlation was obtained with the coronary calcium score. Multiple regression analysis revealed that plasma triglycerides and adiponectin concentrations remained significant independent predictors of apoE-rich (adjusted R-2 = 0.486) but not apoE-poor HDL-C.
Conclusions: The PEG-column method demonstrated, to various degrees, significant correlations between HDL subtractions and several lipid-related biomarkers involved in an atherogenic lipoprotein profile. Our separation technique for apoE-rich HDL is useful to clarify the role of apoE-rich HDL in atherosclerosis. (C) 2016 Elsevier B.V. All rights reserved.
Methods: We established a novel analytical method for apoE-rich HDL using polyethylene glycol and a cation-exchange column (PEG-column method). Furthermore, we examined biochemical correlates of apoE-rich HDL-cholesterol (HDL-C) in 36 patients who underwent coronary computed tomographic angiography.
Results: Our PEG-column method demonstrated high reproducibility (coefficient of variation <3.52%) and linearity up to 15 mg/dl for apoE-rich HDL-C concentrations. Isolated apoE-rich HDL exhibited a larger diameter (14.8 nm) than apoE-poor HDL (10.8 nm) and contained both apoE and apoA-I. ApoE-rich HDL-C concentrations correlated significantly with triglycerides (r(s) = -0.646), LDL size (r(s) = 0.472), adiponectin (r(s) = 0.476), and other lipoprotein components. No significant correlation was obtained with the coronary calcium score. Multiple regression analysis revealed that plasma triglycerides and adiponectin concentrations remained significant independent predictors of apoE-rich (adjusted R-2 = 0.486) but not apoE-poor HDL-C.
Conclusions: The PEG-column method demonstrated, to various degrees, significant correlations between HDL subtractions and several lipid-related biomarkers involved in an atherogenic lipoprotein profile. Our separation technique for apoE-rich HDL is useful to clarify the role of apoE-rich HDL in atherosclerosis. (C) 2016 Elsevier B.V. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.cca.2016.12.016
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/27993510
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000395219100017&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85007495741&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85007495741&origin=inward
- ID情報
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- DOI : 10.1016/j.cca.2016.12.016
- ISSN : 0009-8981
- eISSN : 1873-3492
- PubMed ID : 27993510
- SCOPUS ID : 85007495741
- Web of Science ID : WOS:000395219100017