論文

査読有り 最終著者 責任著者
2018年

ROCK-dependent phosphorylation of NUP62 regulates p63 nuclear transport and squamous cell carcinoma proliferation

EMBO Reports
  • Masaharu Hazawa
  • De-Chen Lin
  • Akiko Kobayashi
  • Yan-Yi Jiang
  • Liang Xu
  • Firli Rahmah Primula Dewi
  • Mahmoud Shaaban Mohamed
  • Hartono
  • Mitsutoshi Nakada
  • Makiko Meguro-Horike
  • Shin-Ichi Horike
  • H Phillip Koeffler
  • Richard W Wong
  • 全て表示

19
1
開始ページ
73
終了ページ
88
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.15252/embr.201744523
出版者・発行元
Wiley-VCH Verlag

p63, more specifically its ΔNp63α isoform, plays essential roles in squamous cell carcinomas (SCCs), yet the mechanisms controlling its nuclear transport remain unknown. Nucleoporins (NUPs) are a family of proteins building nuclear pore complexes (NPC) and mediating nuclear transport across the nuclear envelope. Recent evidence suggests a cell type-specific function for certain NUPs
however, the significance of NUPs in SCC biology remains unknown. In this study, we show that nucleoporin 62 (NUP62) is highly expressed in stratified squamous epithelia and is further elevated in SCCs. Depletion of NUP62 inhibits proliferation and augments differentiation of SCC cells. The impaired ability to maintain the undifferentiated status is associated with defects in ΔNp63α nuclear transport. We further find that differentiation-inducible Rho kinase reduces the interaction between NUP62 and ΔNp63α by phosphorylation of phenylalanine–glycine regions of NUP62, attenuating ΔNp63α nuclear import. Our results characterize NUP62 as a gatekeeper for ΔNp63α and uncover its role in the control of cell fate through regulation of ΔNp63α nuclear transport in SCC.

リンク情報
DOI
https://doi.org/10.15252/embr.201744523
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041864476&origin=inward
ID情報
  • DOI : 10.15252/embr.201744523
  • ISSN : 1469-3178
  • ISSN : 1469-221X
  • SCOPUS ID : 85041864476

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