2018年3月1日
Efficient construction of xenogeneic genomic libraries by circumventing restriction-modification systems that restrict methylated DNA
Journal of Microbiological Methods
- ,
- ,
- 巻
- 146
- 号
- 開始ページ
- 13
- 終了ページ
- 15
- 記述言語
- 英語
- 掲載種別
- 研究論文（学術雑誌）
- DOI
- 10.1016/j.mimet.2018.01.005
- 出版者・発行元
- Elsevier B.V.
An efficient method to construct xenogeneic genomic libraries with low errors and bias by circumventing restriction-modification systems that restrict methylated DNA was developed. Un-methylated genomic DNA of Escherichia coli prepared by ϕ29 DNA polymerase was introduced to Corynebacterium glutamicum R after ligation with un-methylated vector plasmids.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.mimet.2018.01.005
- ISSN : 1872-8359
- ISSN : 0167-7012
- PubMed ID : 29355574
- SCOPUS ID : 85041631042