論文

査読有り
2017年9月

Dynamic DNA methylation reconfiguration during seed development and germination

GENOME BIOLOGY
  • Taiji Kawakatsu
  • ,
  • Joseph R. Nery
  • ,
  • Rosa Castanon
  • ,
  • Joseph R. Ecker

18
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s13059-017-1251-x
出版者・発行元
BIOMED CENTRAL LTD

Background: Unlike animals, plants can pause their life cycle as dormant seeds. In both plants and animals, DNA methylation is involved in the regulation of gene expression and genome integrity. In animals, reprogramming erases and re-establishes DNA methylation during development. However, knowledge of reprogramming or reconfiguration in plants has been limited to pollen and the central cell. To better understand epigenetic reconfiguration in the embryo, which forms the plant body, we compared time-series methylomes of dry and germinating seeds to publicly available seed development methylomes.
Results: Time-series whole genome bisulfite sequencing reveals extensive gain of CHH methylation during seed development and drastic loss of CHH methylation during germination. These dynamic changes in methylation mainly occur within transposable elements. Active DNA methylation during seed development depends on both RNA-directed DNA methylation and heterochromatin formation pathways, whereas global demethylation during germination occurs in a passive manner. However, an active DNA demethylation pathway is initiated during late seed development.
Conclusions: This study provides new insights into dynamic DNA methylation reprogramming events during seed development and germination and suggests possible mechanisms of regulation. The observed sequential methylation/demethylation cycle suggests an important role of DNA methylation in seed dormancy.

リンク情報
DOI
https://doi.org/10.1186/s13059-017-1251-x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000410940600001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1186/s13059-017-1251-x
  • ISSN : 1474-760X
  • Web of Science ID : WOS:000410940600001

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