Ryosuke Kojima

Profile Information

Affiliation: Associate Professor, Graduate School of Medicine, The University of Tokyo

Degree: PhD(The University of Tokyo)

ORCID ID: https://orcid.org/0000-0002-3792-9222
J-GLOBAL ID: 201701009584156404
researchmap Member ID: B000285397

External link: https://scholar.google.com/citations?user=lP4Uu2MAAAAJ&hl=ja

Research Interests

Research Areas

Major Research History

Oct, 2022 - Present

Associate Professor, Graduate School of Medicine, The University of Tokyo
Apr, 2022 - Present

JST FOREST researcher
Jul, 2017 - Sep, 2022

Assistant Professor, Graduate School of Medicine, The University of Tokyo
Oct, 2017 - Mar, 2021

Researcher, JST PRESTO
Jul, 2014 - Jun, 2017

Human Frontier Science Program (HFSP) Long-term Fellow
Jul, 2014 - Jun, 2017

Postdoc, Department of Biosystems Science and Engineering (D-BSSE), Fussenegger lab, ETH Zurich
Apr, 2011 - Mar, 2014

JSPS research fellow (DC1)

Education

- Mar, 2014

Graduate School of Pharmaceutical Sciences, The University of Tokyo
- Mar, 2009

Department of Pharmaceutical Sciences, The University of Tokyo
- Mar, 2005

Komaba-toho High School

Major Awards

Apr, 2023

MEXT Young Scientist Prize
Mar, 2019

Career Development Award,, Human Frontier Science Program (HFSP)

Ryosuke Kojima

Papers

Modular Design Platform for Activatable Fluorescence Probes Targeting Carboxypeptidases Based on ProTide Chemistry

Yugo Kuriki, Mari Sogawa, Toru Komatsu, Minoru Kawatani, Hiroyoshi Fujioka, Kyohhei Fujita, Tasuku Ueno, Kenjiro Hanaoka, Ryosuke Kojima, Rumi Hino …

Journal of the American Chemical Society, Dec 18, 2023
Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis/release regulators

Koki Kunitake, Tadahaya Mizuno, Kazuki Hattori, Chitose Oneyama, Mako Kamiya, Sadao Ota, Yasuteru Urano, Ryosuke Kojima

Sep, 2023 Last authorCorresponding author
Super-resolution vibrational imaging based on photoswitchable Raman probe.

Jingwen Shou, Ayumi Komazawa, Yuusaku Wachi, Minoru Kawatani, Hiroyoshi Fujioka, Spencer John Spratt, Takaha Mizuguchi, Kenichi Oguchi, Hikaru Akaboshi, Fumiaki Obata …

Science advances, 9(24) eade9118, Jun 16, 2023
Development of near-infrared fluorescence probe for the selective detection of severe hypoxia

Takafumi Kasai, Kyohhei Fujita, Toru Komatsu, Tasuku Ueno, Ryosuke Kojima, Kenjiro Hanaoka, Yasuteru Urano

ChemRxiv, May 2, 2023
Activatable Raman Probes Utilizing Enzyme-Induced Aggregate Formation for Selective Ex Vivo Imaging.

Hiroyoshi Fujioka, Minoru Kawatani, Spencer John Spratt, Ayumi Komazawa, Yoshihiro Misawa, Jingwen Shou, Takaha Mizuguchi, Hina Kosakamoto, Ryosuke Kojima, Yasuteru Urano …

Journal of the American Chemical Society, Apr 14, 2023
Rapid imaging of thymoma and thymic carcinoma with a fluorogenic probe targeting γ-glutamyltranspeptidase.

Daisuke Yoshida, Mako Kamiya, Shun Kawashima, Takafusa Yoshioka, Haruaki Hino, Atsuki Abe, Kyohhei Fujita, Ryosuke Kojima, Aya Shinozaki-Ushiku, Yasuteru Urano …

Scientific reports, 13(1) 3757-3757, Mar 7, 2023
A 50 µm acoustic resonator microchannel enables focusing 100 nm polystyrene beads and sub-micron bioparticles

Yoshiyuki Tsuyama, Bin Xu, Kazuki Hattori, Seugho Baek, Yusuke Yoshioka, Ryosuke Kojima, Younghak Cho, Thomas Laurell, Soyoun Kim, Sadao Ota …

Sensors and Actuators B: Chemical, 376 132918-132918, Feb, 2023 Peer-reviewed
9‐Cyano‐10‐telluriumpyronin Derivatives as Red‐light‐activatable Raman Probes

Minoru Kawatani, Spencer J. Spratt, Hiroyoshi Fujioka, Jingwen Shou, Yoshihiro Misawa, Ryosuke Kojima, Yasuteru Urano, Yasuyuki Ozeki, Mako Kamiya

Chemistry – An Asian Journal, Jan 17, 2023
Measuring the pH of Acidic Vesicles in Live Cells with an Optimized Fluorescence Lifetime Imaging Probe.

Kinuko Koda, Sascha Keller, Ryosuke Kojima, Mako Kamiya, Yasuteru Urano

Analytical chemistry, Aug 1, 2022
Droplet Array-Based Platform for Parallel Optical Analysis of Dynamic Extracellular Vesicle Secretion from Single Cells

Kazuki Hattori, Yuki Goda, Minato Yamashita, Yusuke Yoshioka, Ryosuke Kojima, Sadao Ota

Analytical Chemistry, Jul 7, 2022
Rapid imaging of lung cancer using a red fluorescent probe to detect dipeptidyl peptidase 4 and puromycin-sensitive aminopeptidase activities.

Shun Kawashima, Daisuke Yoshida, Takafusa Yoshioka, Akira Ogasawara, Kyohhei Fujita, Masahiro Yanagiya, Masaaki Nagano, Chihiro Konoeda, Haruaki Hino, Kentaro Kitano …

Scientific reports, 12(1) 9100-9100, Jun 1, 2022
Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities.

Yugo Kuriki, Takafusa Yoshioka, Mako Kamiya, Toru Komatsu, Hiroyuki Takamaru, Kyohhei Fujita, Hirohisa Iwaki, Aika Nanjo, Yuki Akagi, Kohei Takeshita …

Chemical science, 13(16) 4474-4481, Apr 20, 2022
Pericentromeric noncoding RNA changes DNA binding of CTCF and inflammatory gene expression in senescence and cancer.

Kenichi Miyata, Yoshinori Imai, Satoshi Hori, Mika Nishio, Tze Mun Loo, Ryo Okada, Liying Yang, Tomoyoshi Nakadai, Reo Maruyama, Risa Fujii …

Proceedings of the National Academy of Sciences of the United States of America, 118(35), Aug 31, 2021
Fluorescence Probes for Imaging Basic Carboxypeptidase Activity in Living Cells with High Intracellular Retention

Hirohisa Iwaki, Mako Kamiya, Minoru Kawatani, Ryosuke Kojima, Tatsuya Yamasoba, Yasuteru Urano

Analytical Chemistry, 93(7) 3470-3476, Feb 23, 2021
γ‐Glutamyltranspeptidase (GGT)‐Activatable Fluorescence Probe for Durable Tumor Imaging

Rui Obara, Mako Kamiya, Yoko Tanaka, Atsuki Abe, Ryosuke Kojima, Tokuichi Kawaguchi, Minoru Sugawara, Akiko Takahashi, Tetsuo Noda, Yasuteru Urano

Angewandte Chemie, 133(4) 2153-2157, Jan 25, 2021
γ-Glutamyltranspeptidase (GGT)-Activatable Fluorescence Probe for Durable Tumor Imaging.

Rui Obara, Mako Kamiya, Yoko Tanaka, Atsuki Abe, Ryosuke Kojima, Tokuichi Kawaguchi, Minoru Sugawara, Akiko Takahashi, Tetsuo Noda, Yasuteru Urano

Angewandte Chemie (International ed. in English), 60(4) 2125-2129, Jan 25, 2021
Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme.

Yasunori Okamoto, Ryosuke Kojima

Methods in molecular biology (Clifton, N.J.), 2312 287-300, 2021
Photoactivatable fluorophores for durable labelling of individual cells

Hiroki Kashima, Mako Kamiya, Fumiaki Obata, Ryosuke Kojima, Shotaro Nakano, Masayuki Miura, Yasuteru Urano

Chemical Communications, 2021
Rapid and Accurate Visualization of Breast Tumors with a Fluorescent Probe Targeting α-Mannosidase 2C1.

Kyohhei Fujita, Mako Kamiya, Takafusa Yoshioka, Akira Ogasawara, Rumi Hino, Ryosuke Kojima, Hiroaki Ueo, Yasuteru Urano

ACS central science, 6(12) 2217-2227, Dec 23, 2020
Multicolor Activatable Raman Probes for Simultaneous Detection of Plural Enzyme Activities.

Hiroyoshi Fujioka, Jingwen Shou, Ryosuke Kojima, Yasuteru Urano, Yasuyuki Ozeki, Mako Kamiya

Journal of the American Chemical Society, 142(49) 20701-20707, Dec 9, 2020

Misc.

合成生物学的アプローチが可能とする細胞外小胞の理解と発展的利用

小嶋良輔, 國武厚貴

生化学, 95(2) 194-200, Apr, 2023
“Designer EV”の開発の現状と将来展望

小嶋良輔

実験医学増刊, 39(20), Dec, 2021 InvitedLead authorCorresponding author
Methods in Molecular Biology, Mammalian Cell Engineering

Ryosuke Kojima (Editor)

May, 2021
遺伝子工学的アプローチを利用したエクソソームのエンジニアリングとその応用

小嶋良輔

膜, 2 62-67, May, 2020 InvitedLead authorCorresponding author
Top Researchers インタビュー

小嶋良輔

Oct, 2019 Invited
特定の生体分子を検知するホタルルシフェリンプローブ

小嶋良輔, 浦野泰照

実験医学別冊発光イメージング実験ガイド, 188-193, Oct, 2019 Invited
特定の細胞を認識してその細胞を殺傷する“Designer Cells” の開発

小嶋良輔

JSMI Report, 12(1) 20-25, Jan, 2019 Peer-reviewedInvited
合成生物学的手法によって改変された”Designer Cells”による新規ドラッグデリバリープラットフォーム

小嶋良輔

日本化学会生体機能関連化学部会ニュースレター, 33(3) 7-14, Dec, 2018 Invited
合成生物学の最前線小嶋良輔

日経バイオテク

Nov, 2018
「次世代医療を目指した細胞間コミュニケーションのエンジニアリング」ETH Zurich、Martin Fussenegger研より

小嶋良輔

Chem-Station, 海外研究記, May, 2018
シグナルタンパク質の物理的な動きを利用して,非免疫細胞に特定の細胞を感知・殺傷させる

小嶋良輔

実験医学, 35(6), Mar, 2018 Invited
Designing bricks for signaling pathways

Ryosuke Kojima

Dec, 2017
非免疫細胞に特定の細胞を殺傷する機能を装備させる

小嶋良輔, Martin Fussenegger

ライフサイエンス新着論文レビュー, Nov, 2017
新規発光制御原理に基づくhROS検出生物発光プローブの開発

小嶋良輔, 高倉栄男, 神谷真子, 小林英司, 長野哲雄, 花岡健二郎, 浦野泰照

2013(7) 35-36, Mar, 2013 Peer-reviewedInvited

Professional Memberships

Major Research Projects

細胞間コミュニケーションの網羅的記録を基軸とした「適応多様性」の理解

科学研究費助成事業, 学術変革領域研究(B), 日本学術振興会, Apr, 2024 - Mar, 2027

小嶋良輔
細胞表面抗原に応答するレポーター酵素スイッチの創製とがん診断・治療への応用

科学研究費助成事業, 基盤研究(B), 日本学術振興会, Apr, 2024 - Mar, 2027

小嶋良輔
合成生物学的手法による細胞外小胞の包括的理解と発展的利用

創発的研究支援事業, JST, Apr, 2022 - Mar, 2025
機能性小分子・タンパク質ペアを利用したがん蛍光イメージングの新手法

科学研究費助成事業基盤研究(B), 基盤研究(B), 日本学術振興会, Apr, 2020 - Mar, 2023

小嶋良輔
Exploiting exosome biology to design polyvalent targeting strategies for in situ cell programming

Career Development Award (CDA), Human Frontier Science Program (HFSP), Mar, 2020 - Mar, 2023

Ryosuke Kojima
In vivo における多対多のアッセイを基盤としたエクソソームターゲティングシステムの効率的探索

戦略的創造研究推進事業さきがけ, 独立行政法人科学技術振興機構, Oct, 2017 - Mar, 2021

小嶋良輔
Synthetis of functional cells capable of rapid modulation of biological systems for future therapies

Long-term Fellow, Human Frontier Science Program (HFSP), Jul, 2014 - Jun, 2017

Ryosuke Kojima
Development of novel luciferin probes that function in specific cells in vivo

Research fellow (DC1), JSPS

Ryosuke Kojima

Industrial Property Rights

WO/2021/095842 Library of barcoded extracellular vesicles
特願2012-020978 New NIR-emitting luciferins

Yasuteru Urano, Tetsuo Nagano, Ryosuke Kojima

Academic Activities

Reviewing Activities

Peer review

Major Other

JSPS fellowship for Research Abroad

Apr, 2014 - Apr, 2014

(Declined)
Research internship at Johns Hopkins University

Jun, 2011 - Sep, 2011

Johns Hopkins University, School of Medicine, Takanari Inoue lab, Research Internship

Profile Information

Research Interests

Research Areas

Major Research History

Education

Major Awards

Papers

Misc.

Professional Memberships

Major Research Projects

Industrial Property Rights

Academic Activities

Major Other

Menu

Coauthors