Mar, 1999
The ability of Vibrio vulnificus to use a synthetic hydrophilic heme compound, Fe-TPPS, as a single iron source
FEMS MICROBIOLOGY LETTERS
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- Volume
- 172
- Number
- 1
- First page
- 73
- Last page
- 77
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/S0378-1097(99)00016-6
- Publisher
- ELSEVIER SCIENCE BV
Vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. This process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin IX). In the present study, we tested whether this pathogen also uses a synthetic heme compound, Fe-alpha,beta,gamma,delta-tetraphenylporphine tetrasulfonic acid (Fe-TPPS), as an iron source. When inoculated into a medium containing Fe-TPPS, V. vulnificus L-180 multiplication was seen to be dependent on the concentration of the synthetic heme compound; a mutant lacking the ability to utilize protoheme did not multiply. Cells of the strain grown under the iron-restricted condition showed time-dependent uptake of Fe-TPPS. The ability to use either protoheme or Fe-TPPS was significantly reduced by the addition of an excess amount of free TPPS or Cu-TPPS. The data suggest that, V. vulnificus may assimilate Fe-TPPS, at least partially, through the same system as that for protoheme. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
- Link information
- ID information
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- DOI : 10.1016/S0378-1097(99)00016-6
- ISSN : 0378-1097
- Web of Science ID : WOS:000078791900012