論文

査読有り
2005年8月

G protein-coupled receptor signaling through Gq and JNK negatively regulates neural progenitor cell migration

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • N Mizuno
  • ,
  • H Kokubu
  • ,
  • M Sato
  • ,
  • A Nishimura
  • ,
  • J Yamauchi
  • ,
  • H Kurose
  • ,
  • H Itoh

102
35
開始ページ
12365
終了ページ
12370
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.0506101102
出版者・発行元
NATL ACAD SCIENCES

In the early development of the central nervous system, neural progenitor cells divide in an asymmetric manner and migrate along the radial glia cells. The radial migration is an important process for the proper lamination of the cerebral cortex. Recently, a new mode of the radial migration was found at the intermediate zone where the neural progenitor cells become multipolar and reduce the migration rate. However, the regulatory signals for the radial migration are unknown. Using the migration assay in vitro, we examined how neural progenitor cell migration is regulated. Neural progenitor cells derived from embryonic mouse telencephalon migrated on laminin-coated dishes. Endothelin (ET)-1 inhibited the neural progenitor cell migration. This ET-1 effect was blocked by BQ788, a specific inhibitor of the ETB receptor, and by the expression of a carboxyl-terminal peptide of G alpha q but not G alpha i. The expression of constitutively active mutant of G alpha q, G alpha qR183C, inhibited the migration of neural progenitor cells. Moreover, the inhibitory effect of ET-1 was suppressed by the c-Jun N-terminal kinase (JNK) inhibitor SP600125 and the expression of the JNK-binding domain of JNK-interacting protein-1, a specific inhibitor of the JNK pathway. Using the slice culture system of embryonic brain, we demonstrated that ET-1 and the constitutively active mutant of G alpha q caused the retention of the neural progenitor cells in the intermediate zone and JNK-binding domain of JNK-interacting protein-1 abrogated the effect of ET-1. These results indicated that G protein-coupled receptor signaling negatively regulates neural progenitor cell migration through Gq and JNK.

リンク情報
DOI
https://doi.org/10.1073/pnas.0506101102
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000231675900017&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.0506101102
  • ISSN : 0027-8424
  • Web of Science ID : WOS:000231675900017

エクスポート
BibTeX RIS