2016年3月
Novel actin filaments from Bacillus thuringiensis form nanotubules for plasmid DNA segregation
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- 巻
- 113
- 号
- 9
- 開始ページ
- E1200
- 終了ページ
- E1205
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1073/pnas.1600129113
- 出版者・発行元
- NATL ACAD SCIENCES
Here we report the discovery of a bacterial DNA-segregating actin-like protein (BtParM) from Bacillus thuringiensis, which forms novel antiparallel, two-stranded, supercoiled, nonpolar helical filaments, as determined by electron microscopy. The BtParM filament features of supercoiling and forming antiparallel double-strands are unique within the actin fold superfamily, and entirely different to the straight, double-stranded, polar helical filaments of all other known ParMs and of eukaryotic F-actin. The BtParM polymers show dynamic assembly and subsequent disassembly in the presence of ATP. BtParR, the DNA-BtParM linking protein, stimulated ATP hydrolysis/phosphate release by BtParM and paired two supercoiled BtParM filaments to form a cylinder, comprised of four strands with inner and outer diameters of 57 angstrom and 145 angstrom, respectively. Thus, in this prokaryote, the actin fold has evolved to produce a filament system with comparable features to the eukaryotic chromosome-segregating microtubule.
- リンク情報
-
- DOI
- https://doi.org/10.1073/pnas.1600129113
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/26873105
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000371204500012&DestApp=WOS_CPL
- URL
- http://orcid.org/0000-0001-6367-6903
- ID情報
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- DOI : 10.1073/pnas.1600129113
- ISSN : 0027-8424
- ORCIDのPut Code : 26727834
- PubMed ID : 26873105
- Web of Science ID : WOS:000371204500012