2015年
CULTURE-INDEPENDENT METHOD FOR SCREENING AND IDENTIFYING MICROBIAL ENZYME-ENCODING GENES USING MICRODROPLET-BASED SINGLE CELL GENOMICS
Proceedings of the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- 開始ページ
- 513
- 終了ページ
- 515
- 記述言語
- 英語
- 掲載種別
- 記事・総説・解説・論説等(国際会議プロシーディングズ)
Environmental microbes are a great source of industrially valuable enzymes with high and unique catalytic activities. However, a vast majority of microbes remain unculturable and thus are not accessible by culture-based methods. Here, we present a rapid and efficient method to screen and identify enzyme-encoding genes from environmental microbes in a culture-independent manner. This method combines activity-based single cell screening using microdroplets and single cell genomics. Using this method, we successfully identified 13 novel β-glucosidase genes from uncultured marine bacteria. This method will facilitate the identification of genes encoding industrially valuable enzymes.
- リンク情報
- ID情報
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- ISBN : 9780979806483
- SCOPUS ID : 84983337885