2014年3月
Evaluation of seizure foci and genes in the Lgi1(L385R+) mutant rat
NEUROSCIENCE RESEARCH
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- 巻
- 80
- 号
- 開始ページ
- 69
- 終了ページ
- 75
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.neures.2013.12.008
- 出版者・発行元
- ELSEVIER IRELAND LTD
Mutations in the leucine-rich, glioma inactivated 1 (LGI1) gene have been identified in patients with autosomal dominant lateral temporal lobe epilepsy (ADLTE). We previously reported that Lgi1 mutant rats, carrying a missense mutation (L385R) generated by gene-driven N-ethyl-N-nitrosourea (ENU) mutagenesis, showed generalized tonic-clonic seizures (GTCS) in response to acoustic stimuli. In the present study, we assessed clinically relevant features of Lgi1 heterozygous mutant rats (Lgi1(L385R/+)) as an animal model of ADLTE. First, to explore the focus of the audiogenic seizures, we performed electroencephalography (EEG) and brain Fos immunohistochemistry in Lgi1(L385R/+) and wild type rats. EEG showed unique seizure patterns (e.g., bilateral rhythmic spikes) in Lgi1(L385R/+) rats with GTCS. An elevated level of Fos expression indicated greater neural excitability to acoustic stimuli in Lgi1(L385R/+) rats, especially in the temporal lobe, thalamus and subthalamic nucleus. Finally, microarray analysis revealed a number of differentially expressed genes that may be involved in epilepsy. These results suggest that Lgi1(L385R/+) rats are useful as an animal model of human ADLTE. (C) 2014 Elsevier Ireland Ltd and the japan Neuroscience Society. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.neures.2013.12.008
- ISSN : 0168-0102
- eISSN : 1872-8111
- PubMed ID : 24406746
- Web of Science ID : WOS:000337013000008