Papers

Peer-reviewed International journal
2018

Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium

Scientific Reports
  • Komori, T.
  • ,
  • Ono, M.
  • ,
  • Hara, E.S.
  • ,
  • Ueda, J.
  • ,
  • Nguyen, H.T.T.
  • ,
  • Nguyen, H.T.
  • ,
  • Yonezawa, T.
  • ,
  • Maeba, T.
  • ,
  • Kimura-Ono, A.
  • ,
  • Takarada, T.
  • ,
  • Momota, R.
  • ,
  • Maekawa, K.
  • ,
  • Kuboki, T.
  • ,
  • Oohashi, T.

Volume
8
Number
1
First page
2612
Last page
2612
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1038/s41598-018-21000-0

Keratinized mucosa is of fundamental importance to maintain healthy gingival tissue, and understanding the mechanisms of oral mucosa keratinization is crucial to successfully manage healthy gingiva. Previous studies have shown a strong involvement of the basement membrane in the proliferation and differentiation of epithelial cells. Therefore, first, to identify the keratinized mucosa-specific basement membrane components, immunohistochemical analysis for the six alpha chains of type IV collagen was performed in 8-week-old mice. No difference in the expression pattern of type IV collagen α1(IV) and α2(IV) chains was observed in the keratinized and non-keratinized mucosa. Interestingly, however, type IV collagen α5(IV) and α6(IV) chains specifically were strongly detected in the keratinized mucosa. To analyze the functional roles of the type IV collagen isoform α6(IV) in oral mucosa keratinization, we analyzed Col4a6-knockout mice. Epithelial developmental delay and low levels of KRT10 were observed in new-born Col4a6-knockout mice. Additionally, in vitro experiments with loss-of function analysis using human gingival epithelial cells confirmed the important role of α6(IV) chain in epithelial keratinization. These findings indicate that α112:α556 (IV) network, which is the only network that includes the α6(IV) chain, is one regulator of KRT10 expression in keratinization of oral mucosal epithelium.

Link information
DOI
https://doi.org/10.1038/s41598-018-21000-0
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29422532
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805778
Scopus Url
http://www.scopus.com/inward/record.url?eid=2-s2.0-85041832414&partnerID=MN8TOARS
ID information
  • DOI : 10.1038/s41598-018-21000-0
  • ORCID - Put Code : 43787899
  • Pubmed ID : 29422532
  • Pubmed Central ID : PMC5805778
  • SCOPUS ID : 85041832414

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