Papers

Peer-reviewed
Nov, 2009

A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) expression by chondrocytes during endochondral ossification

ARCHIVES OF HISTOLOGY AND CYTOLOGY
  • Kanae Kumagishi
  • ,
  • Keiichiro Nishida
  • ,
  • Tomoichiro Yamaai
  • ,
  • Ryusuke Momota
  • ,
  • Shigeru Miyaki
  • ,
  • Satoshi Hirohata
  • ,
  • Ichiro Naito
  • ,
  • Hiroshi Asahara
  • ,
  • Yoshifumi Ninomiya
  • ,
  • Aiji Ohtsuka

Volume
72
Number
3
First page
175
Last page
185
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1679/aohc.72.175
Publisher
INT SOC HISTOLOGY & CYTOLOGY

A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) is known to influence aggrecan degradation in endochondral ossification, but its role has not been well understood. In the present study, in vitro gene expression of ADAMTS9 was investigated by RT-PCR in ATDC5 cells in which experimentally chondrogenic differentiation had been induced. We also investigated the protein localization and gene expression pattern of ADAMTS9 in the tibia growth plate cartilage of male mice in a day 1 neonate, 7-week-old young adult, and a 12-week-old adult by immunohistochemistry and in situ hybridization and compared the results with the expression of proliferating cell nuclear antigen (PCNA) and type X collagen for the identification of proliferative and hypertrophic chondrocyte phenotypes, respectively. We found the gene expression of ADAMTS9 by ATDC5 cells as a dual mode, both before the expression of type X collagen and after hypertrophic differentiation. The immunoreactivity of ADAMTS9 was observed in chondrocytes of proliferative and hypertrophic zones in the growth plate. The population of ADAMTS9 positive cells decreased with age. The results of the present study suggest that ADAMTS9 might have a role in aggrecan cleavage around the chondrocytes to allow chondrocyte proliferation and hypertrophy.


Link information
DOI
https://doi.org/10.1679/aohc.72.175
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20513980
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000277249400004&DestApp=WOS_CPL
URL
http://orcid.org/0000-0002-7003-3444
ID information
  • DOI : 10.1679/aohc.72.175
  • ISSN : 0914-9465
  • eISSN : 1349-1717
  • ORCID - Put Code : 9101211
  • Pubmed ID : 20513980
  • Web of Science ID : WOS:000277249400004

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