Papers

Peer-reviewed
Feb, 2006

In situ preparation of colloidal iron by microwave irradiation for transmission electron microscopy

ACTA MEDICA OKAYAMA
  • S Nakatani
  • ,
  • Naito, I
  • ,
  • R Momota
  • ,
  • N Hinenoya
  • ,
  • K Horiuchi
  • ,
  • K Nishida
  • ,
  • A Ohtsuka

Volume
60
Number
1
First page
59
Last page
64
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.18926/AMO/30753
Publisher
OKAYAMA UNIV MED SCHOOL

We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.


Link information
DOI
https://doi.org/10.18926/AMO/30753
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16508690
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000235538900007&DestApp=WOS_CPL
ID information
  • DOI : 10.18926/AMO/30753
  • ISSN : 0386-300X
  • Pubmed ID : 16508690
  • Web of Science ID : WOS:000235538900007

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