Papers

Peer-reviewed
Feb, 2010

Comparison of Capillary Architecture between Slow and Fast Muscles in Rats Using a Confocal Laser Scanning Microscope

ACTA MEDICA OKAYAMA
  • Shinichiro Murakami
  • ,
  • Hidemi Fujino
  • ,
  • Isao Takeda
  • ,
  • Ryusuke Momota
  • ,
  • Kanae Kumagishi
  • ,
  • Aiji Ohtsuka

Volume
64
Number
1
First page
11
Last page
18
Language
English
Publishing type
Research paper (scientific journal)
Publisher
OKAYAMA UNIV MED SCHOOL

The skeletal muscle is classified into 2 types, slow oxidative or fast glycolytic muscle. For further characterization, we investigated the capillary architecture in slow and fast muscles. The rat soleus and extensor digitorum longus (EDL) muscles were used as representatives of slow and fast muscles, respectively. To investigate capillary density, sections of both types of muscle were stained with alkaline phosphatase; the soleus muscle showed more intense reactivity, indicating that it had a denser capillary structure than the EDL muscle. We then injected fluorescent contrast medium into samples of both muscle types for light and confocal-laser microscopic evaluation. The capillary density and capillary-to-fiber ratio were significantly higher, and the course of the capillaries was more tortuous, in the soleus muscle than in the EDL muscle. Capillary coursed more tortuously in the soleus than in the EDL muscle. Succinate dehydrogenase (SDH) activity, an indicator of mitochondrial oxidative capacity, and vascular endothelial growth factor (VEGF) expression were also significantly higher in the soleus muscle. Thus, we conclude that slow oxidative muscle possess a rich capillary structure to provide demanded oxygen, and VEGF might be involved in the formation and/or maintenance of this highly capillarized architecture.


Link information
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000274868300002&DestApp=WOS_CPL
URL
http://orcid.org/0000-0002-7003-3444
ID information
  • ISSN : 0386-300X
  • ORCID - Put Code : 9101210
  • Web of Science ID : WOS:000274868300002

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