Papers

Peer-reviewed
Dec, 2007

The differential distribution of type IV collagen alpha chains in the subepithelial basement membrane of the human alimentary canal

ARCHIVES OF HISTOLOGY AND CYTOLOGY
  • Hiroyuki Sato
  • ,
  • Ichiro Naito
  • ,
  • Ryusuke Momota
  • ,
  • Yoshio Naomoto
  • ,
  • Tomoki Yamatsuji
  • ,
  • Yoshikazu Sado
  • ,
  • Yoshifumi Ninomiya
  • ,
  • Aiji Ohtsuka

Volume
70
Number
5
First page
313
Last page
323
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1679/aohc.70.313
Publisher
INT SOC HISTOLOGY & CYTOLOGY

We studied distribution patterns of type IV collagen a chains in the subepithelial basement membrane (SBM) of the human gastrointestinal tract - the esophagus through the anal canal - by immunofluorescent microscopy using alpha(IV) chain-specific monoclonal antibodies. The alpha 1(IV), alpha 2(IV), alpha 5(IV), and alpha 6(IV) chains were found in the SBM throughout the tract, indicating the localization of [alpha 1(IV)](2) alpha 2(IV) and [alpha 5(IV)](2) alpha 6(IV) heterotrimeric molecules. The [alpha 1(IV)](2) alpha 2(IV) molecule was continuously stained, while the [alpha 5(IV)]2 alpha 6(IV) molecule was weakly stained in gastric glands and small intestinal crypts. In addition, the SBM at the luminal surface epithelium of the stomach and large intestine contained small amounts of alpha 3(IV) and alpha 4(IV) chains which combined to form the alpha 3(IV) alpha 4(IV) alpha 5(IV) heterotrimeric molecule with alpha 5(IV) chain. The SBM beneath the villous epithelium of the small intestine was also demonstrated to have an alpha 3(IV) chain and alpha 4(IV) chain. Considering the specific locations of the type IV collagen trimers throughout the gastrointestinal SBM, the supramolecular network containing the alpha 3(IV) alpha 4(IV) alpha 5(IV) molecule appears to function as a selective permeability barrier and/or as a protection against chemical stress from the luminal digestive enzymes.


Link information
DOI
https://doi.org/10.1679/aohc.70.313
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18431032
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000255009700006&DestApp=WOS_CPL
URL
http://orcid.org/0000-0002-7003-3444
ID information
  • DOI : 10.1679/aohc.70.313
  • ISSN : 0914-9465
  • eISSN : 1349-1717
  • ORCID - Put Code : 9101214
  • Pubmed ID : 18431032
  • Web of Science ID : WOS:000255009700006

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