Nov, 2007
The distributions of type IV collagen alpha chains in basement membranes of human epidermis and skin appendages
ARCHIVES OF HISTOLOGY AND CYTOLOGY
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- Volume
- 70
- Number
- 4
- First page
- 255
- Last page
- 265
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1679/aohc.70.255
- Publisher
- INT SOC HISTOLOGY & CYTOLOGY
Distributions of type IV collagen a chains in the basement membrane (BM) of human skin and its appendages were analyzed by immunofluorescent microscopy using chain-specific monoclonal antibodies. The basement membrane beneath the epidermis contained [alpha 1(IV)]2 alpha 2(IV) and [ alpha 5(IV)]2 alpha 6(IV) but no alpha 3(IV) alpha 4(IV) alpha 5(IV); this held true for at the eccrine sweat glands and glandular ducts, sebaceous glands, hair follicles, and arrector muscles of hair. The secretary portion of the eccrine sweat glands was rich in [alpha 1(IV)]2 alpha 2(IV) and had less [ alpha 5(IV)]2 alpha 6(IV), while [alpha 5(IV)]2 alpha 6(IV) was abundant in the ductal portion. In the subepidermal zone, alpha 5(IV)/ alpha 6(IV) chain negative spots (1.9-15.0 mu m) were frequently observed. Triple staining samples (Mel.2, alpha 2(IV) and alpha 5(IV) chains) showed that about 50% of epidermal melanocytes colocalized with such spots. Results suggest that these alpha 5(IV)/ alpha 6(IV) chain negative spots of the subepidermal basement membrane have a particular relationship with melanocytes and are sites for certain interactions between the two.
- Link information
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- DOI
- https://doi.org/10.1679/aohc.70.255
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/18296826
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000253206400005&DestApp=WOS_CPL
- URL
- http://orcid.org/0000-0002-7003-3444
- ID information
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- DOI : 10.1679/aohc.70.255
- ISSN : 0914-9465
- eISSN : 1349-1717
- ORCID - Put Code : 9101216
- Pubmed ID : 18296826
- Web of Science ID : WOS:000253206400005